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The chromatographic separation of particles using optical electric fields
Journal article   Peer reviewed

The chromatographic separation of particles using optical electric fields

Nicolas Javier Alvarez, Claus Jeppesen, Kresten Yvind, N. Asger Mortensen and Ole Hassager
Lab on a chip, v 13(5), pp 928-939
01 Jan 2013
PMID: 23325461

Abstract

Biochemical Research Methods Biochemistry & Molecular Biology Chemistry Chemistry, Analytical Chemistry, Multidisciplinary Instruments & Instrumentation Life Sciences & Biomedicine Nanoscience & Nanotechnology Physical Sciences Science & Technology Science & Technology - Other Topics Technology
We introduce a new field-flow fractionation (FFF) technique, whereby molecules are separated based on their differential interaction (dielectrophoresis (DEP)) with optical electric fields, i.e. electric fields with frequencies in the visible and near-infrared range. The results show that a parallel array of axially non-uniform optical fields yielding an attractive potential (positive-DEP-FFF) is advantageous for the separation of polymers, biomolecules, and nanoparticles over very short distances. Furthermore, positive-DEP-FFF yields superior selectivity and resolution compared to conventional separation techniques, which do not lend themselves to miniaturization. A wide range of parameters are considered and the results are presented considering traditional chromatography parameters: the retention ratio and resolution. A simple analytical model is introduced which captures the trends for small normalized decay lengths and will be useful in the design of experimental separation platforms.

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Web of Science research areas
Biochemical Research Methods
Chemistry, Analytical
Chemistry, Multidisciplinary
Instruments & Instrumentation
Nanoscience & Nanotechnology
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