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The effect of chain length on protein solubilization in polymer-based vesicles (polymersomes)
Journal article   Open access   Peer reviewed

The effect of chain length on protein solubilization in polymer-based vesicles (polymersomes)

Veena Pata and Nily Dan
Biophysical journal, v 85(4), pp 2111-2118
Oct 2003
DOI: https://doi.org/10.1016/S0006-3495(03)74639-6
PMID: 14507679
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1016/s0006-3495(03)74639-6View
Published, Version of Record (VoR)Open Access (Publisher-Specific),  Open
url
https://doi.org/10.1016/S0006-3495(03)74639-6View
Published, Version of Record (VoR) Open

Abstract

Amino Acid Sequence Coated Materials, Biocompatible - chemistry Computer Simulation Energy Transfer Inclusion Bodies - chemistry Lipid Bilayers - chemistry Liposomes - chemistry Macromolecular Substances Membrane Fluidity Membrane Proteins - chemistry Models, Chemical Models, Molecular Molecular Sequence Data Molecular Weight Polymers - chemistry Solubility
Using a mean-field analysis we derive a consistent model for the perturbation of a symmetric polymeric bilayer due to the incorporation of transmembrane proteins, as a function of the polymer molecular weight and the protein dimensions. We find that the mechanism for the inhibition of protein incorporation in polymeric bilayers differs from that of their inclusion in polymer-carrying lipid vesicles; in polymersomes, the equilibrium concentration of transmembrane proteins decreases as a function of the thickness mismatch between the protein and the bilayer core, whereas in liposomes the presence of polymer chains affects the protein adsorption kinetics. Despite the increased stiffness of polymer bilayers (when compared to lipid ones), their perturbation decay length and range of protein-protein interaction is found to be relatively long. The energetic penalty due to protein adsorption increases relatively slowly as a function of the polymer chain length due to the self-assembled nature of the polymer bilayer. As a result, we predict that transmembrane proteins may be incorporated in significant numbers even in bilayers where the thickness mismatch is large.

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Web of Science research areas
Biophysics
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