Journal article
The effect of cyclic nucleotides on secretin secretion in canine duodenal mucosa in vitro
Peptides (New York, N.Y. : 1980), v 7(2), pp 357-363
1986
PMID: 2426687
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Abstract
We examined the effects of cyclic nucleotides and calcium on secretin release from canine duodenal mucosal explants incubated in organ culture media. Time course studies revealed that at pH 7.4, 5 and 10 mM dibutyryl cyclic adenosine monophosphate (DBcAMP) increased secretin release progressively, reaching a peak at 2 hours. Two mM of DBcAMP at pH 7.4 did not increase secretin release but at pH 4.5, all 3 doses potentiated secretin release. DBcAMP-stimulated secretin release was not dependent on the influx of extracellular calcium. Graded doses of 3-isobutyl-1-methylxanthine (IBMX) did not stimulate secretin secretion but 1 mM IBMX with 2 mM DBcAMP increased secretin secretion significantly. Dibutyryl cyclic guanosine monophosphate, cholera toxin and 5′-guanylyl-imidodiphosphate (GPP(NH)p) did not stimulate basal secretin release. The release of secretin from our explants incubated at pH 7.4 was not due to specific leakage because all of our viability studies revealed that our explants were functionally intact at the end of 2 hours. Our observations suggest that cyclic nucleotides may participate in the intracellular regulation of secretin secretion.
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Details
- Title
- The effect of cyclic nucleotides on secretin secretion in canine duodenal mucosa in vitro
- Creators
- S.N.S. Murthy - Division of Gastroenterology, Hahnemann University, Philadelphia, PA, USAAlexandra Lavy - Hahnemann University HospitalCraig A. Cassey - Hahnemann University HospitalDavid S. Morgantini - Hahnemann University HospitalVicente P. Dinoso - Hahnemann University HospitalTa-Min Chang - Hahnemann University HospitalGordon T Richards - Physics
- Publication Details
- Peptides (New York, N.Y. : 1980), v 7(2), pp 357-363
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Physics
- Web of Science ID
- WOS:A1986D099700030
- Scopus ID
- 2-s2.0-0022547339
- Other Identifier
- 991019203462404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Endocrinology & Metabolism
- Pharmacology & Pharmacy