Journal article
The effect of divalent cations on the thermostability of type II polyketide synthase acyl carrier proteins
AIChE journal, v 64(12), pp 4308-4318
01 Dec 2018
PMID: 31527922
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The successful engineering of biosynthetic pathways hinges on understanding the factors that influence acyl carrier protein (ACP) stability and function. The stability and structure of ACPs can be influenced by the presence of divalent cations, but how this relates to primary sequence remains poorly understood. As part of a course-based undergraduate research experience, we investigated the thermostability of type II polyketide synthase (PKS) ACPs. We observed an approximate 40 degrees C range in the thermostability among the 14 ACPs studied, as well as an increase in stability (5-26 degrees C) of the ACPs in the presence of divalent cations. Distribution of charges in the helix II-loop-helix III region was found to impact the enthalpy of denaturation. Taken together, our results reveal clues as to how the sequence of type II PKS ACPs relates to their structural stability, information that can be used to study how ACP sequence relates to function. (c) 2018 American Institute of Chemical Engineers
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Details
- Title
- The effect of divalent cations on the thermostability of type II polyketide synthase acyl carrier proteins
- Creators
- Marco A. Rivas - Haverford CollegeValentine C. Courouble - Dept. of Chemistry Haverford College Haverford PA 19041Miranda C. Baker - Dept. of Chemistry Haverford College Haverford PA 19041David L. Cookmeyer - Haverford CollegeKristen E. Fiore - Dept. of Chemistry Haverford College Haverford PA 19041Alexander J. Frost - Dept. of Biology Haverford College Haverford PA 19041Kerilyn N. Godbe - Haverford CollegeMichael R. Jordan - Dept. of Physics Haverford College Haverford PA 19041Emily N. Krasnow - Dept. of Biology Haverford College Haverford PA 19041Aurelio Mollo - Dept. of Chemistry Haverford College Haverford PA 19041Stephen T. Ridings - Dept. of Chemistry Haverford College Haverford PA 19041Keisuke Sawada - Dept. of Chemistry Haverford College Haverford PA 19041Kavita D. Shroff - Dept. of Biology Haverford College Haverford PA 19041Bradley Studnitzer - Dept. of Chemistry Haverford College Haverford PA 19041Grace A. R. Thiele - Dept. of Chemistry Haverford College Haverford PA 19041Ashley C. Sisto - Haverford CollegeSaadia Nawal - Haverford CollegeAdam R. Huff - Haverford CollegeRobert Fairman - Dept. of Biology Haverford College Haverford PA 19041Karl A. Johnson - Dept. of Biology Haverford College Haverford PA 19041Joris Beld - Drexel UniversityBashkim Kokona - Haverford CollegeLouise K. Charkoudian - Haverford College
- Publication Details
- AIChE journal, v 64(12), pp 4308-4318
- Publisher
- Wiley
- Number of pages
- 11
- Grant note
- Haverford College CHE-1652424 / National Science Foundation CAREER Award; National Science Foundation (NSF) 24350 / Cottrell Scholars Award Beckman Foundation R15GM120704 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) R15GM120704 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000449983800018
- Scopus ID
- 2-s2.0-85055120507
- Other Identifier
- 991019168481204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Chemical