Journal article
The effect of moderate hypocapnic ventilation on nuclear Ca2+-ATPase activity, nuclear Ca2+ flux, and Ca2+/calmodulin kinase IV activity in the cerebral cortex of newborn piglets
Neurochemical research, v 29(4), pp 791-796
Apr 2004
PMID: 15098943
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Previous studies have shown that hypocapnia results in fragmentation of nuclear DNA in the cerebral cortex of newborn piglets. We tested the hypothesis that hypocapnia results in decreased ATP and phosphocreatine (PCr) levels and increased nuclear high-affinity Ca++-ATPase activity, intranuclear Ca++ flux, and CaM kinase IV activity in neuronal nuclei of piglets. Three groups of piglets were ventilated as either hypocapnic (a PaCO2 of 20 mm Hg), normocapnic (a PaCO2 of 40 mm Hg), or corrected hypocapnic (ventilated as hypocapnic but with CO2 added to maintain normocapnia) for 1 h. Tissue ATP levels were lower in the hypocapnic than in the normocapnic group. PCr levels were lower and 45Ca++-influx, Ca++-ATPase activity and CaM kinase IV activity were higher in hypocapnic than in normocapnic or corrected hypocapnic piglets. We conclude that hypocapnia alters nuclear membrane Ca++ flux mechanisms and may alter neuronal phosphorylation mechanisms in the cerebral cortex of piglets.
Metrics
Details
- Title
- The effect of moderate hypocapnic ventilation on nuclear Ca2+-ATPase activity, nuclear Ca2+ flux, and Ca2+/calmodulin kinase IV activity in the cerebral cortex of newborn piglets
- Creators
- Karen I Fritz - Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, Neonatology Research Laboratories, Philadelphia,Alan B Zubrow - Drexel UniversityQazi M Ashraf - Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, Neonatology Research Laboratories, Philadelphia,Om P Mishra - Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, Neonatology Research Laboratories, Philadelphia,Maria Delivoria-Papadopoulos - Drexel University
- Publication Details
- Neurochemical research, v 29(4), pp 791-796
- Grant note
- HD-38079 / NICHD NIH HHS HL-07027 / NHLBI NIH HHS HD-20337 / NICHD NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:000220086000017
- Scopus ID
- 2-s2.0-1842559733
- Other Identifier
- 991019169579204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences