Journal article
The effect of the elapsed time between blood draw and processing on the recovery of fetal cells from maternal blood
Journal of the Society for Gynecologic Investigation, v 11(3), pp 154-165
01 Apr 2004
PMID: 15051035
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
OBJECTIVETo test the hypothesis that a delay in initial fetal cell enrichment processing of maternal blood samples (defined as the time between blood draw and the initial density gradient centrifugation step) compromises the ability to recover fetal cells, we performed a randomized comparison of immediate (within 4 hours of draw) versus delayed (between 18-24 hours of draw) processing.METHODSFour centers participated: two centers utilized flow cytometry (FLOW), and two centers utilized magnetic-activated cell sorting (MACS) techniques. Each center collected 34 samples. The outcome was the percentage of gamma positive (gamma(+)) cells for FLOW or the number of nucleated red blood cells (NRBCs) for MACS, found in the final enriched cell population. Both outcomes reflect cell properties that are potentially fetal in origin, thus making them representative of the ability to recover fetal cells.RESULTSOur results did not support our hypothesis that delay in processing compromises fetal cell recovery. Instead, in MACS processing, we observed an increase in recovered NRBCs when blood sample processing was delayed compared with immediate processing. There was no significant difference in gamma(+) cells with FLOW.CONCLUSIONTime-related changes in the density of target cells, perhaps associated with their progress towards apoptosis during the delay period, may result in increased intact fetal cells with the study methods utilized.
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Details
- Title
- The effect of the elapsed time between blood draw and processing on the recovery of fetal cells from maternal blood
- Creators
- K A Dukes - DM-STAT (United States)L M Sullivan - DM-STAT Inc., Medford, USAD Lewis - DM-STAT (United States)K L Johnson - DM-STAT (United States)D W Bianchi - DM-STAT Inc., Medford, USAJ L Simpson - DM-STAT Inc., Medford, USAW Holzgreve - DM-STAT Inc., Medford, USAS Hahn - DM-STAT Inc., Medford, USAF Z Bischoff - DM-STAT Inc., Medford, USAL G Jackson - DM-STAT Inc., Medford, USA
- Publication Details
- Journal of the Society for Gynecologic Investigation, v 11(3), pp 154-165
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Education
- Web of Science ID
- WOS:000220813400005
- Scopus ID
- 2-s2.0-12144288126
- Other Identifier
- 991019169804304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Obstetrics & Gynecology