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The effects of glucose and an aldose reductase inhibitor on the sorbitol content and collagen synthesis of bovine retinal capillary pericytes in culture
Journal article   Peer reviewed

The effects of glucose and an aldose reductase inhibitor on the sorbitol content and collagen synthesis of bovine retinal capillary pericytes in culture

Weiye Li, Mahin Khatami and John H. Rockey
Experimental eye research, v 40(3), pp 439-444
1985
PMID: 3933992

Abstract

aldose reductase inhibitor collagen synthesis high glucose retinal capillary pericytes sorbitol
The absolute rate of collagen synthesis by cultured bovine retinal capillary pericytes, determined using the specific radioactivity of proline in the cellular amino acid pool, was compared in media containing different concentrations of glucose (5, 10 or 40 m m) and Sorbinil (0·0 or 0·1 m M), an inhibitor of aldose reductase. The absolute rate of collagen synthesis, in proline molar terms, by pericytes in medium with 5 m m glucose was 3·3 ± 1·9 ( s.d.) pmol 10 −7 cells 24 hr −1, and increased significantly to 8·8 ± 3·7 pmol 10 −7 cells 24 hr −1 when the glucose concentration was increased to 40 m m. Sorbinil (0·1 m m) reduced the elevated sorbitol contents of pericytes induced by high concentrations of glucose, but did not significantly change the absolute rate of collagen synthesis per cell.

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Ophthalmology
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