Journal article
The expression of CD4 by T cell precursors resident in both the thymus and the bone marrow
The Journal of immunology (1950), v 151(9), pp 4486-4493
01 Nov 1993
PMID: 7691951
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A very small fraction of thymocytes has recently been identified that expresses low levels of CD4 in the absence of CD8, CD3, or a TCR. These CD4lo thymocytes appear to be the precursors of the early CD4-CD8-CD3- thymic subset and contain most of the T cell progenitor activity found within the thymus. Here, we examined adult bone marrow for the presence of a similar population of cells and found that 0.5% to 3.5% of C58/J bone marrow cells express low, but detectable levels of CD4 (CD4lo) at the cell surface in the absence of CD3. These CD4lo bone marrow cells display pre-T cell activity, in that they are able to repopulate the thymus of irradiated recipient mice after intrathymic transfer. Moreover, we found that most of pre-T cell activity found in the bone marrow is contained within the CD4lo expressing subset of marrow cells. Although the CD4lo cells found in both the thymus and bone marrow display pre-T cell activity, the CD4lo cells from these two sites showed pronounced differences with respect to their ability to respond to specific cytokine stimulation in vitro. Bone marrow-resident CD4lo cells proliferated in response to both IL-3 and mast cell growth factor in vitro, whereas CD4lo cells isolated from the thymus did not. Furthermore, CD4lo bone marrow cells, grown in media containing IL-3 and mast cell growth factor, retained their pre-T cell activity, indicating that CD4lo cells with pre-T cell capabilities were among the IL-3 and mast cell growth factor-responsive cells. These data suggest that although pre-T cells in bone marrow share the CD4lo phenotype with their intrathymic counterparts, they may be fundamentally different with respect to the environmental factors that control their growth.
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Details
- Title
- The expression of CD4 by T cell precursors resident in both the thymus and the bone marrow
- Creators
- R Chervenak - Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130D DempseyR SoloffR M WolcottS R Jennings
- Publication Details
- The Journal of immunology (1950), v 151(9), pp 4486-4493
- Publisher
- United States
- Grant note
- R01 AI31567 / NIAID NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1993MD34900007
- Scopus ID
- 2-s2.0-0027364340
- Other Identifier
- 991014878621404721
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- Web of Science research areas
- Immunology