Journal article
The extent of sequence complementarity correlates with the potency of cellular miRNA-mediated restriction of HIV-1
Nucleic acids research, v 40(22), pp 11684-11696
01 Dec 2012
PMID: 23042677
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
MicroRNAs (miRNAs) are 22-nt non-coding RNAs involved in the regulation of cellular gene expression and potential cellular defense against viral infection. Using in silico analyses, we predicted target sites for 22 human miRNAs in the HIV genome. Transfection experiments using synthetic miRNAs showed that five of these miRNAs capably decreased HIV replication. Using one of these five miRNAs, human miR-326 as an example, we demonstrated that the degree of complementarity between the predicted viral sequence and cellular miR-326 correlates, in a Dicer-dependent manner, with the potency of miRNA-mediated restriction of viral replication. Antagomirs to miR-326 that knocked down this cell endogenous miRNA increased HIV-1 replication in cells, suggesting that miR-26 is physiologically functional in moderating HIV-1 replication in human cells.
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Details
- Title
- The extent of sequence complementarity correlates with the potency of cellular miRNA-mediated restriction of HIV-1
- Creators
- Laurent Houzet - National Institute of Allergy and Infectious DiseasesZachary Klase - University of Hong KongMan Lung Yeung - University of Hong KongAnnie Wu - University of Hong KongShu-Yun Le - NCI, Ctr Canc Res Nanobiol Program, NCI Ctr Canc Res, Frederick, MD 21702 USAMariam Quinones - University of Hong KongKuan-Teh Jeang - NIAID, Mol Virol Sect, Mol Microbiol Lab, NIH, Bethesda, MD 20892 USA
- Publication Details
- Nucleic acids research, v 40(22), pp 11684-11696
- Publisher
- Oxford Univ Press
- Number of pages
- 13
- Grant note
- Office of AIDS Research; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Intramural NIAID funds Intramural AIDS Targeted Antiviral Program (IATAP) from the Office of the Director, NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA NIH (NIAID intramural fund) ZIAAI000547 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000313414800051
- Scopus ID
- 2-s2.0-84870342928
- Other Identifier
- 991021902599004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology