Journal article
The genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4
Environmental microbiology, v 13(12), pp 3289-3309
Dec 2011
PMID: 21951522
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Bacillus pseudofirmus
OF4 is an extreme but facultative alkaliphile that grows non-fermentatively in a pH range from 7.5 to above 11.4 and can withstand large sudden increases in external pH. It is a model organism for studies of bioenergetics at high pH, at which energy demands are higher than at neutral pH because both cytoplasmic pH homeostasis and ATP synthesis require more energy. The alkaliphile also tolerates a cytoplasmic pH > 9.0 at external pH values at which the pH homeostasis capacity is exceeded, and manages other stresses that are exacerbated at alkaline pH, e.g. sodium, oxidative and cell wall stresses. The genome of
B. pseudofirmus
OF4 includes two plasmids that are lost from some mutants without viability loss. The plasmids may provide a reservoir of mobile elements that promote adaptive chromosomal rearrangements under particular environmental conditions. The genome also reveals a more acidic p
I
profile for proteins exposed on the outer surface than found in neutralophiles. A large array of transporters and regulatory genes are predicted to protect the alkaliphile from its overlapping stresses. In addition, unanticipated metabolic versatility was observed, which could ensure requisite energy for alkaliphily under diverse conditions.
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Details
- Title
- The genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4
- Creators
- Benjamin Janto - Allegheny General Hospital, Allegheny-Singer Research Institute, Center for Genomic Sciences and Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania 15212Azad Ahmed - Allegheny General Hospital, Allegheny-Singer Research Institute, Center for Genomic Sciences and Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania 15212Masahiro Ito - Graduate School of Life Sciences, Toyo University, Ora-gun, Gunma 374-0193, JapanJun Liu - Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029David B Hicks - Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029Sarah Pagni - Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029Oliver J Fackelmayer - Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029Terry-Ann Smith - Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029Joshua Earl - Allegheny General Hospital, Allegheny-Singer Research Institute, Center for Genomic Sciences and Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania 15212Liam D.H Elbourne - Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia 2109Karl Hassan - Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia 2109Ian T Paulsen - Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia 2109Anne-Brit Kolstø - Laboratory for Microbial Dynamics and Department of Pharmaceutical Biosciences, University of Oslo, Blindern 0316 Oslo, NorwayNicolas J Tourasse - Institut de Biologie Physico-Chimique, Université Pierre et Marie Curie, 75005 Paris, FranceGarth D Ehrlich - Allegheny General Hospital, Allegheny-Singer Research Institute, Center for Genomic Sciences and Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania 15212Robert Boissy - Allegheny General Hospital, Allegheny-Singer Research Institute, Center for Genomic Sciences and Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania 15212D. Mack Ivey - Department of Biological Sciences, University of Arkansas, Fayetteville, Arkansas 7270Gang Li - Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P. R. ChinaYanfen Xue - Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P. R. ChinaYanhe Ma - Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P. R. ChinaFen Z Hu - Allegheny General Hospital, Allegheny-Singer Research Institute, Center for Genomic Sciences and Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania 15212Terry A Krulwich - Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 10029
- Publication Details
- Environmental microbiology, v 13(12), pp 3289-3309
- Publisher
- Wiley
- Grant note
- R01 GM028454-27 || GM / National Institute of General Medical Sciences : NIGMS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Neurobiology and Anatomy
- Web of Science ID
- WOS:000298843800018
- Scopus ID
- 2-s2.0-82555205330
- Other Identifier
- 991014877785204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Microbiology