Journal article
The hNT human neuronal cell line survives and migrates into rat retina
Cell transplantation, v 7(6), pp 549-558
1998
PMID: 9853583
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The present studies were undertaken to determine if hNT cells can survive in the vitreous of the eye and migrate into the retina. The hNT neuronal cell line represents a uniform source of human tissue that may be of use in retinal grafts. hNT cells stored in liquid nitrogen were thawed and labeled with the fluorescent dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). Thirty thousand cells in 1 μL were injected epiretinally in rat. At survival times of 3, 14, 28, or 56 days, retinal sections were examined quantitatively by epifluorescence to reveal DiI-labeled cells. hNT cells survived in the vitreous at all time points without evidence of vascularization. At 3 days, essentially no hNT cells were found in deep retina, and only very few were attached to retina. At days 14, 28, and 56, hNT cells were found to cluster on the vitreal/retinal interface, and in deeper layers. The clusters of hNT cells took on the shape of a funnel at 14 days, and inverted funnel at 28 days, and by 56 days, populated the photoreceptor layer as a stratum. It is possible that hNT cells took on the morphology and function of photoreceptors. These results suggest that hNT cells injected epiretinally survive in the vitreous at least 56 days, migrate to the retinal/vitreous interface, and may migrate through the retina. This system permits the independent and quantitative evaluation of survival and migratory trophic responses.
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Details
- Title
- The hNT human neuronal cell line survives and migrates into rat retina
- Creators
- Toshifumi Konobu - Nara Medical University, Kashihara, Nara, JapanFrancis Sessler - Department of Neurobiology and Anatomy, Allegheny University of the Health Sciences, Philadelphia, PA, USALai-yi Luo - Department of NeurosurgeryAllegheny University of the Health Sciences, Philadelphia, PA, USAJohn Lehmann - Department of NeurosurgeryAllegheny University of the Health Sciences, Philadelphia, PA, USA
- Publication Details
- Cell transplantation, v 7(6), pp 549-558
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000077167900005
- Scopus ID
- 2-s2.0-0031794855
- Other Identifier
- 991014877948504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell & Tissue Engineering
- Medicine, Research & Experimental
- Transplantation