Journal article
The hepatitis B virus HBx protein modulates cell cycle regulatory proteins in cultured primary human hepatocytes
Virus research, v 155(1), pp 363-367
2011
PMID: 20934470
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
There are over 350 million people chronically infected with the Hepatitis B virus (HBV); chronic HBV infections are associated with the development of hepatocellular carcinoma (HCC). While the precise mechanism of HBV-associated HCC remains undefined, it is believed to involve a combination of the host immune response to infection and activities of HBV proteins including the nonstructural X protein (HBx). HBx is a multifunctional protein that can modulate various cellular processes including cell proliferation. The exact effect of HBx on cell proliferation has varied depending on the cell line and exact conditions used in the study. Our previously published reports have demonstrated that HBx modulates the levels of cell cycle regulatory proteins in primary rat hepatocytes; however, the effect of HBx on cell cycle regulatory proteins in primary human hepatocytes, the natural host for HBV infection, has not been studied. Here we have examined the effect of HBx on cell cycle regulatory proteins in cultured, primary human hepatocytes. We demonstrate that HBx decreases the levels of cell cycle proteins that prevent progression into G1 phase and increases the levels of cell cycle proteins active in G1 phase. We have also shown that HBx modulation of cell cycle regulatory proteins requires cytosolic calcium, similar to the results we previously obtained in primary rat hepatocytes. Cumulatively, our results are the first demonstration that HBx modulates the levels of cell cycle regulatory proteins in a calcium-dependent manner in primary human hepatocytes.
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Details
- Title
- The hepatitis B virus HBx protein modulates cell cycle regulatory proteins in cultured primary human hepatocytes
- Creators
- Tricia L Gearhart - Graduate Program in Molecular and Cellular Biology and Genetics, Drexel University College of Medicine, Philadelphia, PA 19102, United StatesMichael J Bouchard - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N. 15th Street, Philadelphia, PA 19102, United States
- Publication Details
- Virus research, v 155(1), pp 363-367
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000287010300049
- Scopus ID
- 2-s2.0-78650575534
- Other Identifier
- 991014877824504721
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- Web of Science research areas
- Virology