Journal article
The immune response to a vesicular stomatitis virus vaccine vector is independent of particulate antigen secretion and protein turnover rate
Journal of virology, v 86(8), pp 4253-4261
Apr 2012
PMID: 22345454
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Vesicular stomatitis virus (VSV) is a highly cytopathic virus being developed as a vaccine vector due to its ability to induce strong protective T cell and antibody responses after a single dose. However, little is known regarding the mechanisms underlying the potent immune responses elicited by VSV. We previously generated a VSV vector expressing the hepatitis B virus middle envelope surface glycoprotein (MS) that induces strong MS-specific T cell and antibody responses in mice. After synthesis in the cytoplasm, the MS protein translocates to the endoplasmic reticulum, where it forms subviral particles that are secreted from the cell. To better understand the contributions of secreted and intracellular protein to the VSV-induced immune response, we produced a vector expressing a secretion-deficient MS mutant (MS(C69A)) and compared the immunogenicity of this vector to that of the wild-type VSV-MS vector in mice. As expected, the MS(C69A) protein was not secreted from VSV-infected cells and displayed enhanced proteasome-mediated degradation. Surprisingly, despite these differences in intracellular protein processing, the T cell and antibody responses generated to MS(C69A) were comparable to those elicited by virus expressing wild-type MS protein. Therefore, when it is expressed from VSV, the immune responses to MS are independent of particulate antigen secretion and the turnover rate of cytoplasmic protein. These results are consistent with a model in which the immune responses to VSV are strongly influenced by the replication cycle of the vector and demonstrate that characteristics of the vector have the capacity to affect vaccine efficacy more than do the properties of the antigen itself.
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Details
- Title
- The immune response to a vesicular stomatitis virus vaccine vector is independent of particulate antigen secretion and protein turnover rate
- Creators
- Melissa A Cobleigh - Yale UniversityClinton Bradfield - Yale UniversityYuanjie Liu - bDrexel Institute for Biotechnology and Virology Research, Doylestown, Pennsylvania, USAAnand Mehta - bDrexel Institute for Biotechnology and Virology Research, Doylestown, Pennsylvania, USAMichael D Robek - Yale University
- Publication Details
- Journal of virology, v 86(8), pp 4253-4261
- Publisher
- American Society for Microbiology (ASM)
- Grant note
- T32GM00732 / NIGMS NIH HHS R01 CA131133 / NCI NIH HHS CA131133 / NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000302185400021
- Scopus ID
- 2-s2.0-84861385675
- Other Identifier
- 991019318935104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Virology