Journal article
The inability of human immunodeficiency virus to infect chimpanzee monocytes can be overcome by serial viral passage in vivo
Journal of virology, v 65(7), pp 3853-3863
Jul 1991
PMID: 1674968
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Studies of lentivirus infection in ruminants, nonhuman primates, and humans suggest that virus infection of macrophages plays a central role in the disease process. To investigate whether human immunodeficiency virus type 1 (HIV-1) can infect chimpanzee macrophages, we recovered monocytes from peripheral blood mononuclear cells of HIV-1-negative animals and inoculated these and control human monocytes with a panel of four human-passaged monocytotropic virus strains and one chimpanzee-passaged isolate. HIV-1 infected human monocytes synthesized proviral DNA, viral mRNA, p24 antigen, and progeny virions. In contrast, except for the chimpanzee-passaged HIV-1 isolate, chimpanzee monocytes failed to support HIV-1 replication when cultured under both identical and a variety of other conditions. Proviral DNA was demonstrated only at background levels in these cell cultures by polymerase chain reaction for gag- and env-related sequences. Interestingly, the chimpanzee-passaged HIV-1 isolate did not replicate in human monocytes; viral p24 antigens and progeny virions were not detected. The same monocytotropic panel of HIV-1 strains replicated in both human and chimpanzee CD4+ T lymphoblasts treated with phytohemagglutinin and interleukin-2. The failure of HIV-1 to infect chimpanzee monocytes, which can be overcome by serial in vivo viral passage, occurs through a block early in the viral life cycle.
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Details
- Title
- The inability of human immunodeficiency virus to infect chimpanzee monocytes can be overcome by serial viral passage in vivo
- Creators
- H E Gendelman - Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, Maryland 20852G D Ehrlich - Henry M. Jackson FoundationL M Baca - Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, Maryland 20852S Conley - Henry M. Jackson FoundationJ Ribas - Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, Maryland 20852D C Kalter - Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, Maryland 20852M S Meltzer - Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, Maryland 20852B J Poiesz - Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, Maryland 20852P Nara - Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, Maryland 20852
- Publication Details
- Journal of virology, v 65(7), pp 3853-3863
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1991FQ94000053
- Scopus ID
- 2-s2.0-0025861695
- Other Identifier
- 991019196783004721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Virology