Journal article
The influence of printing parameters on cell survival rate and printability in microextrusion-based 3D cell printing technology
Biofabrication, v 7(4), pp 045002/1-045002/11
02 Nov 2015
PMID: 26523399
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Three-dimensional (3D) cell printing technology has provided a versatile methodology to fabricate cell-laden tissue-like constructs and in vitro tissue pathological models for tissue engineering, drug testing and screening applications. However, it still remains a challenge to print bioinks with high viscoelasticity to achieve long-term stable structure and maintain high cell survival rate after printing at the same time. In this study, we systematically investigated the influence of 3D cell printing parameters, i.e. composition and concentration of bioink, holding temperature and holding time, on the printability and cell survival rate in microextrusion-based 3D cell printing technology. Rheological measurements were utilized to characterize the viscoelasticity of gelatin-based bioinks. Results demonstrated that the bioink viscoelasticity was increased when increasing the bioink concentration, increasing holding time and decreasing holding temperature below gelation temperature. The decline of cell survival rate after 3D cell printing process was observed when increasing the viscoelasticity of the gelatin-based bioinks. However, different process parameter combinations would result in the similar rheological characteristics and thus showed similar cell survival rate after 3D bioprinting process. On the other hand, bioink viscoelasticity should also reach a certain point to ensure good printability and shape fidelity. At last, we proposed a protocol for 3D bioprinting of temperature-sensitive gelatin-based hydrogel bioinks with both high cell survival rate and good printability. This research would be useful for biofabrication researchers to adjust the 3D bioprinting process parameters quickly and as a referable template for designing new bioinks.
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Details
- Title
- The influence of printing parameters on cell survival rate and printability in microextrusion-based 3D cell printing technology
- Creators
- Yu Zhao - Tsinghua UniversityYang Li - Tsinghua UniversityShuangshuang Mao - Tsinghua UniversityWei Sun - Tsinghua UniversityRui Yao - Tsinghua University
- Publication Details
- Biofabrication, v 7(4), pp 045002/1-045002/11
- Publisher
- IOP Publishing
- Number of pages
- 11
- Grant note
- 51235006 / National Natural Science Foundation of China (http://dx.doi.org/10.13039/501100001809) 2012AA020506 / National High Technology Research and Development Program of China
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000366896900008
- Scopus ID
- 2-s2.0-84953924038
- Other Identifier
- 991019167710604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials