The promotion of in vitro vessel-like organization of endothelial cells in magnetically responsive alginate scaffolds

Yulia Sapir; Smadar Cohen; Gary Friedman; Boris Polyak

doi:10.1016/j.biomaterials.2012.02.037

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The promotion of in vitro vessel-like organization of endothelial cells in magnetically responsive alginate scaffolds

Journal article

Open access

Peer reviewed

The promotion of in vitro vessel-like organization of endothelial cells in magnetically responsive alginate scaffolds

Yulia Sapir, Smadar Cohen, Gary Friedman and Boris Polyak

Biomaterials, v 33(16), pp 4100-4109

Jun 2012

DOI: https://doi.org/10.1016/j.biomaterials.2012.02.037

PMID: 22417620

Featured in Collection : UN Sustainable Development Goals @ Drexel

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https://europepmc.org/articles/pmc3565424View

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Abstract

Alginate scaffold

Endothelial cell organization

Magnetic nanoparticles

Magneto-mechanical cell stimulation

Vessel-like structures

One of the major challenges in engineering thick, complex tissues such as cardiac muscle, is the need to pre-vascularize the engineered tissue in vitro to enable its efficient integration with host tissue upon implantation. Herein, we explored new magnetic alginate composite scaffolds to provide means of physical stimulation to cells. Magnetite-impregnated alginate scaffolds seeded with aortic endothelial cells stimulated during the first 7 days out of a total 14 day experimental course showed significantly elevated metabolic activity during the stimulation period. Expression of proliferating cell nuclear antigen (PCNA) indicated that magnetically stimulated cells had a lower proliferation index as compared to the non-stimulated cells. This suggests that the elevated metabolic activity could instead be related to cell migration and re-organization. Immunostaining and confocal microscopy analyses supported this observation showing that on day 14 in magnetically stimulated scaffolds without supplementation of any growth factors, cellular vessel-like (loop) structures, known as indicators of vasculogenesis and angiogenesis were formed as compared to cell sheets or aggregates observed in the non-stimulated (control) scaffolds. This work is the first step in our understanding of how to accurately control cellular organization to form tissue engineered constructs, which together with additional molecular signals could lead to a creation of an efficient pre-vascularized tissue construct with potential applicability for transplantation.

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Details

Title: The promotion of in vitro vessel-like organization of endothelial cells in magnetically responsive alginate scaffolds
Creators: Yulia Sapir - Ben-Gurion University of the Negev
Smadar Cohen - Ben-Gurion University of the Negev
Gary Friedman - Drexel University
Boris Polyak - Drexel University
Publication Details: Biomaterials, v 33(16), pp 4100-4109
Publisher: Elsevier
Resource Type: Journal article
Language: English
Academic Unit: Electrical and Computer Engineering; Surgery
Web of Science ID: WOS:000303096300009
Scopus ID: 2-s2.0-84858793272
Other Identifier: 991019169598804721

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Collaboration types: Domestic collaboration; International collaboration
Web of Science research areas: Engineering, Biomedical; Materials Science, Biomaterials