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The protein kinase CK2 substrate Jabba modulates lipid metabolism during Drosophila oogenesis
Journal article   Open access   Peer reviewed

The protein kinase CK2 substrate Jabba modulates lipid metabolism during Drosophila oogenesis

Emily A McMillan, Sheila M Longo, Michael D Smith, Sarah Broskin, Baicheng Lin, Nisha K Singh and Todd I Strochlic
The Journal of biological chemistry, v 293(8), pp 2990-3002
23 Feb 2018
DOI: https://doi.org/10.1074/jbc.M117.814657
PMID: 29326167
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1074/jbc.m117.814657View
Published, Version of Record (VoR)CC BY V4.0,  Open
url
https://doi.org/10.1074/jbc.M117.814657View
Published, Version of Record (VoR) Open

Abstract

3T3-L1 Cells Animals Animals, Genetically Modified Carrier Proteins - chemistry Carrier Proteins - genetics Carrier Proteins - metabolism Casein Kinase II - antagonists & inhibitors Casein Kinase II - chemistry Casein Kinase II - genetics Casein Kinase II - metabolism Crosses, Genetic Drosophila melanogaster - genetics Drosophila Proteins - antagonists & inhibitors Drosophila Proteins - chemistry Drosophila Proteins - genetics Drosophila Proteins - metabolism Female Gene Expression Regulation, Developmental Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism HEK293 Cells Humans Lipid Metabolism Mice Microscopy, Fluorescence Oogenesis Ovary - cytology Ovary - enzymology Ovary - metabolism Peptide Fragments - chemistry Peptide Fragments - genetics Peptide Fragments - metabolism Recombinant Fusion Proteins - metabolism RNA Interference Substrate Specificity
Lipid metabolism plays a critical role in female reproduction. During oogenesis, maturing oocytes accumulate high levels of neutral lipids that are essential for both energy production and the synthesis of other lipid molecules. Metabolic pathways within the ovary are partially regulated by protein kinases that link metabolic status to oocyte development. Although the functions of several kinases in this process are well established, the roles that many other kinases play in coordinating metabolic state with female germ cell development are unknown. Here, we demonstrate that the catalytic activity of casein kinase 2 (CK2) is essential for oogenesis. Using an unbiased biochemical screen that leveraged an unusual catalytic property of the kinase, we identified a novel CK2 substrate in the ovary, the lipid droplet-associated protein Jabba. We show that Jabba is essential for modulating ovarian lipid metabolism and for regulating female fertility in the fly. Our findings shed light on a CK2-dependent signaling pathway governing lipid metabolism in the ovary and provide insight into the long-recognized but poorly understood association between energy metabolism and female reproduction.

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15 citations in Web of Science
14 citations in Scopus

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Web of Science research areas
Biochemistry & Molecular Biology
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