Journal article
The relationship between the trimethylation of lysine 77 and cytochrome c metabolism in Saccharomyces cerevisiae
International journal of biochemistry, v 26(5), pp 721-734
1994
PMID: 8005357
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Abstract
1.
1. Site directed mutations were constructed in the yeast iso-1-cytochrome
c gene adjacent to the lysine 77 (methylation site) codon.
2.
2. These mutant genes were then cloned and transformed into the
S. cerevisiae strain B-6642 which contains a deficiency in the iso-1-cytochrome
c gene.
3.
3. The resulting transformants were screened for cytochrome
c production using gel electrophoresis.
4.
4. Amino acid analysis of the mutated cytochromes
c demonstrated varying levels of trimethyllysine formation, depending on the nature of the site directed mutation.
5.
5. The resulting transformants were then used as tools in order to investigate the relationship between trimethyllysine formation and various aspects of cytochrome
c metabolism including protein stability and heme conjugation.
Metrics
Details
- Title
- The relationship between the trimethylation of lysine 77 and cytochrome c metabolism in Saccharomyces cerevisiae
- Creators
- Karamba J CeesayLynn R RiderLawrence W BergmanMartin T Tuck
- Publication Details
- International journal of biochemistry, v 26(5), pp 721-734
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1994NL14400013
- Scopus ID
- 2-s2.0-0028176792
- Other Identifier
- 991014878482804721
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- Web of Science research areas
- Biochemistry & Molecular Biology