Journal article
The resistance of DMC1 D-loops to dissociation may account for the DMC1 requirement in meiosis
Nature structural & molecular biology, v 18(1)
Jan 2011
PMID: 21151113
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The ubiquitously expressed Rad51 and the meiosis-specific Dmc1 recombinases promote the formation of strand invasion products (D-loops) between homologous molecules. Strand invasion products are processed by either the double strand break repair (DSBR) or synthesis-dependent strand annealing (SDSA) pathway. D-loops destined to being processed by SDSA need to dissociate producing noncrossovers (NCOs) and those destined for DSBR should resist dissociation to generate crossovers (COs). The mechanism that channels recombination intermediates into different HR pathways is unknown. Here we demonstrate that D-loops in a DMC1 driven reaction are substantially more resistant to dissociation by branch migration proteins such as RAD54, than those formed by RAD51. We propose that the intrinsic resistance to dissociation of DMC1 strand invasion intermediates may account for why DMC1 is essential to ensure the proper segregation of chromosomes in meiosis.
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Details
- Title
- The resistance of DMC1 D-loops to dissociation may account for the DMC1 requirement in meiosis
- Creators
- Dmitry V Bugreev - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102-1192, USARoberto J Pezza - Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, Bethesda, Maryland 20892, USAOlga M Mazina - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102-1192, USAOleg N Voloshin - Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, Bethesda, Maryland 20892, USAR. Daniel Camerini-Otero - Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, Bethesda, Maryland 20892, USAAlexander V Mazin - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102-1192, USA
- Publication Details
- Nature structural & molecular biology, v 18(1)
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000285966800009
- Scopus ID
- 2-s2.0-78650983490
- Other Identifier
- 991014878073904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics
- Cell Biology