Journal article
The role of the promoter in the expression of the PCNA gene
Gene expression, v 2(3), pp 285-296
24 Jul 2018
PMID: 1360287
Abstract
G
1
-specific temperature-sensitive (ts) mutants of the cell cycle arrest in G
1
after serum stimulation at the restrictive temperature. Under these conditions, the RNA levels of late growth-regulated genes (such as DNA polymerase alpha, PCNA, thymidine kinase, and core histones) are markedly decreased or even undetectable, while early growth-regulated genes (for instance, c-myc) are normally expressed, and certain promoters are actually super-induced. We have used the human PCNA gene transfected into TK
−
tsl3 cells (a G
1
-specific ts mutant) to investigate whether the inhibition of gene expression caused by this type of growth inhibition occurs at a transcriptional or posttranscriptional level. Constructs were made in which the 5′ and 3′ flanking sequences of the human PCNA gene were replaced by the corresponding elements of the SV40 T antigen coding gene. Using these constructs and data from run-on assays and RT-PCR, we conclude that the failure of expression of the PCNA gene in G
1
-arrested TK
−
tsl3 cells occurs at the transcriptional level.
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Details
- Title
- The role of the promoter in the expression of the PCNA gene
- Creators
- Renee-Helene Charollais - Thomas Jefferson UniversityHansjuerg Alder - Thomas Jefferson UniversityAndres Ferber - Thomas Jefferson UniversityJanice Koniecki - Thomas Jefferson UniversityChristian Sell - Thomas Jefferson UniversityRenato Baserga - Thomas Jefferson University
- Publication Details
- Gene expression, v 2(3), pp 285-296
- Publisher
- Cognizant Communication Corporation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Scopus ID
- 2-s2.0-0027031802
- Other Identifier
- 991020110940904721