Journal article
The secretory phenotype of senescent astrocytes isolated from Wistar newborn rats changes with anti-inflammatory drugs, but does not have a short-term effect on neuronal mitochondrial potential
Biogerontology (Dordrecht), v 19(5), pp 415-433
Oct 2018
PMID: 30097900
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In the central nervous system (CNS), senescent astrocytes have been associated with neurodegeneration. Senescent cells secrete a complex mixture of pro-inflammatory factors, which are collectively called Senescence Associated Secretory Phenotype (SASP). The SASP components can vary depending on the cell type, senescence inducer and time. The SASP has been mainly studied in fibroblasts and epithelial cells, but little is known in the context of the CNS. Here, the SASP profile in senescent astrocytes isolated from Wistar newborn rats induced to senescence by oxidative stress or by proteasome inhibition was analyzed. Senescent astrocytes secreted predominantly chemokines and IL-1α, but no IL-6. The effect of the anti-inflammatory drugs, sulforaphane (SFN) and dehydroepiandrosterone (DHEA), on the SASP profile was evaluated. Our results showed that SFN and DHEA decreased IL-1α secretion while increasing IL-10, thus modifying the SASP to a less anti-inflammatory profile. Primary neurons were subjected to the conditioned media obtained from drug-treated senescent astrocytes, and their mitochondrial membrane potential was evaluated.
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Details
- Title
- The secretory phenotype of senescent astrocytes isolated from Wistar newborn rats changes with anti-inflammatory drugs, but does not have a short-term effect on neuronal mitochondrial potential
- Creators
- Luis Ángel Maciel-Barón - Universidad Autónoma MetropolitanaSandra Lizbeth Morales-Rosales - Universidad Autónoma MetropolitanaAlejandro Silva-Palacios - Universidad Autónoma MetropolitanaRoxana Haydee Rodríguez-Barrera - Universidad Anáhuac México NorteJorge Antonio García-Álvarez - National Autonomous University of MexicoArmando Luna-López - Instituto Nacional de PediatriaViviana Isabel Pérez - Linus Pauling InstituteClaudio Torres - Drexel UniversityMina Königsberg - Universidad Autónoma Metropolitana
- Publication Details
- Biogerontology (Dordrecht), v 19(5), pp 415-433
- Publisher
- Springer Nature
- Grant note
- Red Temática de Investigación en Salud y Desarrollo Social / CONACYT FON.INST/298/2016 / CONACYT
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000442597100007
- Scopus ID
- 2-s2.0-85051293128
- Other Identifier
- 991019169640804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Geriatrics & Gerontology