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The transport of Mycobacterium avium subsp. paratuberculosis through saturated aquifer materials
Journal article   Peer reviewed

The transport of Mycobacterium avium subsp. paratuberculosis through saturated aquifer materials

C H Bolster, K L Cook, B Z Haznedaroglu and S L Walker
Letters in applied microbiology, v 48(3), 307
Mar 2009
PMID: 19207858

Abstract

Animals Bacterial Adhesion DNA, Bacterial - analysis DNA, Bacterial - isolation & purification Fresh Water - microbiology Geologic Sediments - microbiology Hydrophobic and Hydrophilic Interactions Mycobacterium avium subsp. paratuberculosis - genetics Mycobacterium avium subsp. paratuberculosis - isolation & purification Mycobacterium avium subsp. paratuberculosis - physiology Surface Properties Water Movements
To investigate the processes controlling the transport of Mycobacterium avium subsp. paratuberculosis (Map) through aquifer materials. We measured two important surface characteristics known to affect bacterial attachment to sediment surfaces: surface charge and hydrophobicity. We then measured the transport of Map through laboratory columns packed with aquifer sand with varying ionic strength solutions and sediment surface charge. We found that Map has a strong negative charge and is highly hydrophobic and that the transport of Map through positively charged Fe-coated sands was reduced compared with transport through negatively charged clean quartz sand, although Map transport for all treatments was low compared with the transport behaviour reported in the literature for other bacteria. Our results suggest that the potential for groundwater contamination by Map is low; however, the organism may remain bound to the soil near the surface where it can be ingested by grazing animals or be released during run off events. This is the first study looking at the surface characteristics and transport behaviour of Map through aquifer materials and therefore provides important information for understanding the movement of Map in the environment.

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Collaboration types
Domestic collaboration
Web of Science research areas
Biotechnology & Applied Microbiology
Microbiology
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