Journal article
The two-component system ScnRK of Streptococcus mutans affects hydrogen peroxide resistance and murine macrophage killing
Microbes and infection, v 10(3), pp 293-301
01 Mar 2008
PMID: 18316220
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To survive macrophage killing is critical in the pathogenesis of viridians streptococci-induced infective endocarditis (IE).
Streptococcus mutans, an opportunistic IE pathogen, generally does not survive well phagocytic killing in murine macrophage RAW 264.7 cells. A putative two-component system (TCS), ScnR/ScnK from
S. mutans, was investigated to elucidate the mechanisms underlying bacteria-cellular interaction in this study. Both the wild-type and mutant strains were phagocytosed by RAW 264.7 cells at a comparable rate and an increased intracellular susceptibility during a 5
h incubation period was observed with the
scnRK-null mutants. The amount of reactive oxygen species (ROS) in activated macrophages was reduced significantly after ingesting wild-type, but not
scnRK-null mutant strains, suggesting that increased macrophage killing of these mutants is due to the impaired ability of
S. mutans to counteract ROS. Additionally, both
scnR- or
scnRK-null mutants were more susceptible to hydrogen peroxide. Interestingly,
scnRK expression was unaffected by hydrogen peroxide. These experimental results indicate that
scnRK is important in counteracting oxidative stress in
S. mutans, and decreased susceptibility to phagocytic killing is at least partly attributable to inhibition of intracellular ROS formation.
Metrics
Details
- Title
- The two-component system ScnRK of Streptococcus mutans affects hydrogen peroxide resistance and murine macrophage killing
- Creators
- Pei-Min Chen - National Taiwan UniversityHeng-Chang Chen - National Taiwan UniversityChun-Ta Ho - National Taiwan UniversityChiau-Jing Jung - National Taiwan UniversityHuei-Ting Lien - National Taiwan UniversityJen-Yang Chen - National Health Research Institute, No. 135, Keyan Road, Zhunan Town, Miaoli County 35053, Taiwan, ROCJean-San Chia - National Taiwan University
- Publication Details
- Microbes and infection, v 10(3), pp 293-301
- Publisher
- Elsevier SAS
- Number of pages
- 9
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000255672300011
- Scopus ID
- 2-s2.0-41049103061
- Other Identifier
- 991022008195504721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology
- Infectious Diseases
- Microbiology