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Published, Version of Record (VoR)CC BY-NC-ND V4.0, Open
Journal article
Three-Dimensional Reconstruction of Activated Columns from 2-[ 14C]Deoxy- d-glucose Data
NeuroImage (Orlando, Fla.), v 2(1), pp 9-20
1995
PMID: 9343586
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Three-dimensional (3-D) reconstruction of autoradiograms can provide new insights into the functional relationship of neural regions. To reach full potential, however, 3-D reconstruction must be both accurate and efficient. In this paper, we present a novel image matching algorithm that simultaneously aligns a set of serial sections and uses the method to reconstruct whisker barrels from the rat cerebral cortex. We initially compared several alignment techniques and found that our Multi-Set Registration (MSR) algorithm produced superior accuracy. This algorithm is based on a least-squares minimization technique and is able to simultaneously register a set of serial sections with subpixel precision (30-μm accuracy). We applied our new technique to the 3-D reconstruction of a series of autoradiograms. Our objective was to visualize and measure the 3-D metabolic (functional) shape of normal (control) and developmentally altered (plastic) C3 vibrissa columns in the first somatosensory area of the rat cerebral cortex. The plastic C3 metabolic column showed a nearly 450% increase in volume when compared to the control column. In addition, the lesion-altered C3 column—in contrast to the normal C3 column—displayed no central zone of high activity, and patches of higher metabolic activity were scattered throughout the columnar profile. This metabolic activity was not confined to the cylindrical column, but extended tangentially as radiating fingerlike projections toward neighboring barrels.
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Details
- Title
- Three-Dimensional Reconstruction of Activated Columns from 2-[ 14C]Deoxy- d-glucose Data
- Creators
- Alberto F. Goldszal - Drexel UniversityOleh J. Tretiak - Drexel UniversityPeter J. Hand - Imaging and Computer Vision Center, Drexel University, Philadelphia, Pennsylvania 19104 and Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104Sanjay Bhasin - Imaging and Computer Vision Center, Drexel University, Philadelphia, Pennsylvania 19104 and Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104Donald L. McEachron - Drexel University
- Publication Details
- NeuroImage (Orlando, Fla.), v 2(1), pp 9-20
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; [Retired Faculty]
- Web of Science ID
- WOS:A1995QY54000003
- Scopus ID
- 2-s2.0-0029079148
- Other Identifier
- 991019173748304721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neuroimaging
- Neurosciences
- Radiology, Nuclear Medicine & Medical Imaging