Journal article
Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation
eLife, v 2(2), pp e01008-e01008
03 Sep 2013
PMID: 24015360
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
During cell locomotion and endocytosis, membrane-tethered WASP proteins stimulate actin filament nucleation by the Arp2/3 complex. This process generates highly branched arrays of filaments that grow toward the membrane to which they are tethered, a conflict that seemingly would restrict filament growth. Using three-color single-molecule imaging in vitro we revealed how the dynamic associations of Arp2/3 complex with mother filament and WASP are temporally coordinated with initiation of daughter filament growth. We found that WASP proteins dissociated from filament-bound Arp2/3 complex prior to new filament growth. Further, mutations that accelerated release of WASP from filament-bound Arp2/3 complex proportionally accelerated branch formation. These data suggest that while WASP promotes formation of pre-nucleation complexes, filament growth cannot occur until it is triggered by WASP release. This provides a mechanism by which membrane-bound WASP proteins can stimulate network growth without restraining it. DOI:http://dx.doi.org/10.7554/eLife.01008.001.
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Details
- Title
- Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation
- Creators
- Benjamin A Smith - Brandeis UniversityShae B Padrick - Howard Hughes Medical InstituteLynda K Doolittle - Howard Hughes Medical InstituteKaren Daugherty-Clarke - Brandeis UniversityIvan R Corrêa, JrMing-Qun XuBruce L Goode - Brandeis UniversityMichael K Rosen - Howard Hughes Medical InstituteJeff Gelles - Brandeis University
- Publication Details
- eLife, v 2(2), pp e01008-e01008
- Publisher
- eLife
- Grant note
- R01 GM098143 / NIGMS NIH HHS GM43369 / NIGMS NIH HHS R01 GM063691 / NIGMS NIH HHS Howard Hughes Medical Institute GM63691 / NIGMS NIH HHS GM098143 / NIGMS NIH HHS T32 GM007596 / NIGMS NIH HHS R01 GM056322 / NIGMS NIH HHS GM56322 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000328626200007
- Scopus ID
- 2-s2.0-84884697646
- Other Identifier
- 991020836326904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biology