Journal article
Three-dimensional bio-printing of primary human hepatocellular carcinoma for personalized medicine
Biomaterials, v 265, 120416
Jan 2021
PMID: 33007612
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Hepatocellular carcinoma (HCC) is one of the most lethal tumors worldwide. This study aims to address the lack of faithful and available in vitro models for patient-specific drug screening for HCC. We recently established a novel modeling system using three-dimensional (3D) bioprinting technology and constructed hepatorganoids with HepaRG cells, which retain the liver function and prolong the survival of mice with liver failure after abdominal transplantation. Here we extend this modeling system to establish individualized model for hepatocellular carcinoma. HCC specimens were obtained from six patients after surgery. Primary HCC cells were isolated and mixed with gelatin and sodium alginate to form the bioink for printing. Patient-derived three-dimensional bio-printed HCC (3DP-HCC) models were successfully established afterward and grew well during long-term culture. These models retained the features of parental HCCs, including stable expression of the biomarker, stable maintenances of the genetic alterations and expression profiles. 3DP-HCC models are capable of displaying the results of drug screening intuitively and quantitatively. In conclusion, 3DP-HCC models are faithful in vitro models that are reliable in long-term culture and able to predict patient-specific drugs for personalized treatment.
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Details
- Title
- Three-dimensional bio-printing of primary human hepatocellular carcinoma for personalized medicine
- Creators
- Feihu Xie - Peking Union Medical College HospitalLejia Sun - Peking Union Medical College HospitalYuan Pang - Tsinghua UniversityGang Xu - Peking Union Medical College HospitalBao Jin - Peking Union Medical College HospitalHaifeng Xu - Peking Union Medical College HospitalXin Lu - Peking Union Medical College HospitalYiyao Xu - Peking Union Medical College HospitalShunda Du - Peking Union Medical College HospitalYanan Wang - State Key Laboratory of Medical Molecular Biology, Department of Physiology, Institute of Basic Medical Sciences, PUMC & CAMS, Beijing, 100005, China.Shi Feng - Peking Union Medical College HospitalXinting Sang - Peking Union Medical College HospitalShouxian Zhong - Peking Union Medical College HospitalXin Wang - University of MinnesotaWei Sun - Drexel UniversityHaitao Zhao - Peking Union Medical College HospitalHongbing Zhang - State Key Laboratory of Medical Molecular Biology, Department of Physiology, Institute of Basic Medical Sciences, PUMC & CAMS, Beijing, 100005, China.Huayu Yang - Peking Union Medical College HospitalPengyu Huang - Center for Excellence in Molecular Cell ScienceYilei Mao - Peking Union Medical College Hospital
- Publication Details
- Biomaterials, v 265, 120416
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000591612700004
- Scopus ID
- 2-s2.0-85091630988
- Other Identifier
- 991019167467804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials