Journal article
Three-dimensional microfluidic tumor-macrophage system for breast cancer cell invasion
Biotechnology and bioengineering, v 116(7), pp 1731-1741
01 Jul 2019
PMID: 30802293
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The recrudescence of breast cancer can partly be attributed to poor understanding of the early steps and the mechanisms involved in breast cancer metastasis, especially how tumor inflammatory cells including tumor-associated macrophages (TAM) affect invasion process. However, invasion-related biological studies in traditional in vitro assays or in vivo models are challenging due to the arduousness in establishing models that precisely reproduce the tumor invasion environment. To this end, we proposed a juxtaposed dual-layer cell-loaded hydrogels biomimetic microfluidic system and formed monolayer size-selective permeable vascular endothelial barriers besides the dual layer to mimic mammalian blood vessels. We clarified that in this system, TAM promoted the invasion of breast cancer cells, whereas breast cancer cells maintained the phenotype of TAM cells and promoted the differentiation of U937 cells into TAM. It formed a tumor-macrophage bidirectional crosstalk system. This system could be used for drug screening. So finally, through the calculation of the survival rate of breast cancer cells when cocultured with different macrophages under paclitaxel treatment, we analyzed the antagonism of tumor-macrophage bidirectional crosstalk on anticancer drugs.
Metrics
Details
- Title
- Three-dimensional microfluidic tumor-macrophage system for breast cancer cell invasion
- Creators
- Shengli Mi - Tsinghua UniversityZhaoyu Liu - Tsinghua Univ, Open FIESTA Ctr, Shenzhen, Peoples R ChinaZhichang Du - University Town of ShenzhenXiaoman Yi - Tsinghua Univ, Grad Sch Shenzhen, Shenzhen, Peoples R ChinaWei Sun - University Town of Shenzhen
- Publication Details
- Biotechnology and bioengineering, v 116(7), pp 1731-1741
- Publisher
- Wiley
- Number of pages
- 11
- Grant note
- JCYJ20170412101508433; JCYJ20160608165954934; JCYJ20160509154841455; JCYJ20160509154951210 / Shenzhen Basic Research Projects
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000469929000015
- Scopus ID
- 2-s2.0-85062947842
- Other Identifier
- 991019167704004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology