Journal article
Three-dimensional printing of Hela cells for cervical tumor model in vitro
Biofabrication, v 6(3), pp 035001/1-035001/10
01 Sep 2014
PMID: 24722236
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Advances in three-dimensional (3D) printing have enabled the direct assembly of cells and extracellular matrix materials to form in vitro cellular models for 3D biology, the study of disease pathogenesis and new drug discovery. In this study, we report a method of 3D printing for Hela cells and gelatin/alginate/fibrinogen hydrogels to construct in vitro cervical tumor models. Cell proliferation, matrix metalloproteinase (MMP) protein expression and chemoresistance were measured in the printed 3D cervical tumor models and compared with conventional 2D planar culture models. Over 90% cell viability was observed using the defined printing process. Comparisons of 3D and 2D results revealed that Hela cells showed a higher proliferation rate in the printed 3D environment and tended to form cellular spheroids, but formed monolayer cell sheets in 2D culture. Hela cells in 3D printed models also showed higher MMP protein expression and higher chemoresistance than those in 2D culture. These new biological characteristics from the printed 3D tumor models in vitro as well as the novel 3D cell printing technology may help the evolution of 3D cancer study.
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Details
- Title
- Three-dimensional printing of Hela cells for cervical tumor model in vitro
- Creators
- Yu Zhao - Tsinghua UniversityRui Yao - Tsinghua UniversityLiliang Ouyang - Tsinghua UniversityHongxu Ding - Tsinghua UniversityTing Zhang - Tsinghua UniversityKaitai Zhang - Peking Union Medical College HospitalShujun Cheng - Peking Union Medical College HospitalWei Sun (Author) - Tsinghua University
- Publication Details
- Biofabrication, v 6(3), pp 035001/1-035001/10
- Publisher
- Iop Publishing Ltd
- Number of pages
- 10
- Grant note
- 2012AA020506 / National High Technology Research and Development Program of China (863); National High Technology Research and Development Program of China NSF-CMMI-1030520 / National Science Foundation; National Science Foundation (NSF) 51235006 / Chinese National Natural Science Foundation; National Natural Science Foundation of China (NSFC)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000341823500001
- Scopus ID
- 2-s2.0-84899560969
- Other Identifier
- 991019167711304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials