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Journal article
Three-dimensional, three-vector-component velocimetry of cilia-driven fluid flow using correlation-based approaches in optical coherence tomography
Biomedical optics express, v 6(9), pp 3515-3538
24 Aug 2015
PMID: 26417520
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Microscale quantification of cilia-driven fluid flow is an emerging area in medical physiology, including pulmonary and central nervous system physiology. Cilia-driven fluid flow is most completely described by a three-dimensional, three-component (3D3C) vector field. Here, we generate 3D3C velocimetry measurements by synthesizing higher dimensional data from lower dimensional measurements obtained using two separate optical coherence tomography (OCT)-based approaches: digital particle image velocimetry (DPIV) and dynamic light scattering (DLS)-OCT. Building on previous work, we first demonstrate directional DLS-OCT for 1D2C velocimetry measurements in the sub-1 mm/s regime (sub-2.5 inch/minute regime) of cilia-driven fluid flow in
Xenopus
epithelium, an important animal model of the ciliated respiratory tract. We then extend our analysis toward 3D3C measurements in
Xenopus
using both DLS-OCT and DPIV. We demonstrate the use of DPIV-based approaches towards flow imaging of
Xenopus
cerebrospinal fluid and mouse trachea, two other important ciliary systems. Both of these flows typically fall in the sub-100
μ
m/s regime (sub-0.25 inch/minute regime). Lastly, we develop a framework for optimizing the signal-to-noise ratio of 3D3C flow velocity measurements synthesized from 2D2C measures in non-orthogonal planes. In all, 3D3C OCT-based velocimetry has the potential to comprehensively characterize the flow performance of biological ciliated surfaces.
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Details
- Title
- Three-dimensional, three-vector-component velocimetry of cilia-driven fluid flow using correlation-based approaches in optical coherence tomography
- Creators
- Brendan K. Huang - Yale UniversityUte A. Gamm - Yale UniversityVineet Bhandari - Yale UniversityMustafa K. Khokha - Yale UniversityMichael A. Choma - Yale University
- Publication Details
- Biomedical optics express, v 6(9), pp 3515-3538
- Publisher
- Optical Society of America
- Grant note
- 1R01HD081379; NIH MSTP TG T32GM07205; NIH1R01HL118419-01; NIH1R21HL120783; NIHR01HL085103 / National Institutes of Health (NIH) Basil O’Connor Starter Scholar Research Award / March of Dimes Foundation (March of Dimes Births Defect Foundation)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:000360888400035
- Scopus ID
- 2-s2.0-84948737731
- Other Identifier
- 991019173526404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Optics
- Radiology, Nuclear Medicine & Medical Imaging