Journal article
Thrombospondin-1 Is Elevated with both Intimal Hyperplasia and Hypercholesterolemia
The Journal of surgical research, v 74(1), pp 11-16
Jan 1998
PMID: 9536966
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background.Thrombospondin-1 (TSP-1) is important in platelet adhesion and aggregation, inflammation, cell to cell interaction, angiogenesis, and smooth muscle cell (SMC) proliferation. TSP-1 expression increases rapidly with injury. Therefore, we hypothesize that TSP-1 may play a role in the development of intimal hyperplasia (IH). The purpose of this study is to examine the interaction between cholesterol and TSP-1 on SMC proliferation and to quantitatively assess TSP-1 expression in an established model of IH, with and without underlying cholesterol-induced atherosclerosis.
Materials and methods. In vitro,rabbit aortic SMC culture studies were performed to see the effect of TSP-1 antibodies on PDGF and, separately, cholesterol-induced SMC proliferation.In vivo,23 rabbits were fed either a regular or a high-cholesterol diet. Hypercholesterolemia was confirmed by measurement of serum levels. Subsets underwent intraluminal aortic injury. Aortas were harvested 8–10 weeks later. Arterial wall TSP-1 was evaluated immunohistochemically and quantified by computer image analysis.
Results. In vitro,TSP-1 antibodies were able to inhibit PDGF and cholesterol-induced SMC proliferation (P< 0.05).In vivo,TSP-1 was found predominantly in the extracellular matrix in the rabbit aorta. IH was uniformly seen status-post angioplasty. Hyperplasia was more prominent in samples from hypercholesterolemic animals. ANOVA and Student'sttest analyses demonstrated significantly more TSP-1 in the high-cholesterol/angioplasty group than in all other groups (P= 0.0006 vs regular diet/no angioplasty group).
Conclusions.These data are consistent with the hypothesis that TSP-1 contributes to the development of IH. This study suggests that injured arteries in hypercholesterolemic atherosclerotic rabbits overexpress TSP-1.
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Details
- Title
- Thrombospondin-1 Is Elevated with both Intimal Hyperplasia and Hypercholesterolemia
- Creators
- Jeffrey J. Roth - Allegheny University of the Health SciencesVivian Gahtan - Allegheny University of the Health SciencesJeffrey L. Brown - Allegheny University of the Health SciencesChristine Gerhard - Allegheny University of the Health SciencesVanlila K. Swami - Allegheny University of the Health SciencesVicki L. Rothman - Allegheny University of the Health SciencesThomas N. Tulenko - Allegheny University of the Health SciencesGeorge P. Tuszynski - Allegheny University of the Health Sciences
- Publication Details
- The Journal of surgical research, v 74(1), pp 11-16
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:000073373600004
- Scopus ID
- 2-s2.0-0031845732
- Other Identifier
- 991019167323104721
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- Web of Science research areas
- Surgery