Journal article
Thrombospondin-1 and transforming growth factor-betal promote breast tumor cell invasion through up-regulation of the plasminogen/plasmin system
Surgery, v 122(2), pp 493-500
1997
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background. Pericellular proteolysis is crucial in tumor cell invasion. The plasminogen/plasmin system is one of the main protease systems involved in cancer progression. Thrombospondin-1 (TSP-1), through activation of transforming growth factor-β1 (TGF-β1), up-regulates the main plasminogen activator, the urokinase-type plasminogen activator (uPA). The objectives of this study were to determine the role of TSP-1 and TGF-β1 in the localization of the plasminogen/plasmin system to the tumor cell surface by the uPA receptor (uPAR) and to determine its effect in breast tumor cell invasion.
Methods. The effect of TSP-1 and TGF-β1 in uPAR expression was determined in MDA-MB-231 human breast cancer cells by enzyme-linked immunosorbent assay and Western blot analysis. Their effect and the role of the plasminogen/plasmin system in breast tumor cell invasion were studied with a Boyden Chamber assay.
Results. uPAR expression was up-regulated more than twofold by both TSP-1 and TGF-β1. The effect of TSP-1 involved its receptor and the activation of TGF-β1 by TSP-1. Breast tumor cell invasion was up-regulated sevenfold to eightfold by both TSP-1 and TGF-β1 compared with the control group. Antibodies against uPA or uPAR neutralized the TSP-1- and TGF-β1-promoted breast tumor cell invasion.
Conclusions. TSP-1, through the activation of endogenous TGF-β1, up-regulates the plasminogen/plasmin system and promotes tumor cell invasion in breast cancer cells.
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Details
- Title
- Thrombospondin-1 and transforming growth factor-betal promote breast tumor cell invasion through up-regulation of the plasminogen/plasmin system
- Creators
- Daniel Albo - Allegheny University of the Health SciencesDavid H Berger - Allegheny University of the Health SciencesThomas N Wang - Allegheny University of the Health SciencesXiaolong Hu - Allegheny University of the Health SciencesVicki Rothman - Allegheny University of the Health SciencesGeorge P Tuszynski - Allegheny University of the Health Sciences
- Publication Details
- Surgery, v 122(2), pp 493-500
- Publisher
- Mosby, Inc
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Information Science
- Web of Science ID
- WOS:A1997XU46300086
- Scopus ID
- 2-s2.0-0030868422
- Other Identifier
- 991019167341904721
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- Surgery