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Journal article
Ti3C2Tx MXene Flakes for Optical Control of Neuronal Electrical Activity
ACS nano, v 15(9), pp 14662-14671
28 Sep 2021
PMID: 34431659
Abstract
Understanding cellular electrical communications in both health and disease necessitates precise subcellular electrophysiological modulation. Nanomaterial-assisted photothermal stimulation was demonstrated to modulate cellular activity with high spatiotemporal resolution. Ideal candidates for such an application are expected to have high absorbance at the near-infrared window, high photothermal conversion efficiency, and straightforward scale-up of production to allow future translation. Here, we demonstrate two-dimensional Ti3C2Tx (MXene) as an outstanding candidate for remote, nongenetic, optical modulation of neuronal electrical activity with high spatiotemporal resolution. Ti3C2Tx's photothermal response measured at the single-flake level resulted in local temperature rises of 2.31 +/- 0.03 and 3.30 +/- 0.02 K for 635 and 808 nm laser pulses (1 ms, 10 mW), respectively. Dorsal root ganglion (DRG) neurons incubated with Ti3C2Tx film (25 mu g/cm(2)) or Ti3C2Tx flake dispersion (100 mu g/mL) for 6 days did not show a detectable influence on cellular viability, indicating that Ti3C2Tx is noncytotoxic. DRG neurons were photothermally stimulated using Ti3C2Tx films and flakes with as low as tens of microjoules per pulse incident energy (635 nm, 2 mu J for film, 18 mu J for flake) with subcellular targeting resolution. Ti3C2Tx's straightforward and large-scale synthesis allows translation of the reported photothermal stimulation approach in multiple scales, thus presenting a powerful tool for modulating electrophysiology from single-cell to additive manufacturing of engineered tissues.
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Details
- Title
- Ti3C2Tx MXene Flakes for Optical Control of Neuronal Electrical Activity
- Creators
- Yingqiao Wang - Carnegie Mellon UniversityRaghav Garg - Carnegie Mellon UniversityJane E. Hartung - University of PittsburghAdam Goad - Drexel UniversityDipna A. Patel - Drexel UniversityFlavia Vitale - Center for Neurotrauma, Neurodegeneration, and Restoration, Corporal Michael J. Crescenz Veterans Affairs Medical Center, Philadelphia, Pennsylvania 19104, United States.Michael S. Gold - University of PittsburghYury Gogotsi - Drexel UniversityTzahi Cohen-Karni - Carnegie Mellon University
- Publication Details
- ACS nano, v 15(9), pp 14662-14671
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 10
- Grant note
- MCF-677785 / Department of Materials Science and Engineering Materials Characterization Facility AWD00001593 (416052-5) / Defense Advanced Research Projects Agency; United States Department of Defense; Defense Advanced Research Projects Agency (DARPA) K12HD073945 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA CBET1552833; DMR-1740795 / National Science Foundation; National Science Foundation (NSF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Materials Science and Engineering
- Web of Science ID
- WOS:000703553600063
- Scopus ID
- 2-s2.0-85114617621
- Other Identifier
- 991019169559904721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology