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Time-dependent nanomechanics of cartilage
Journal article   Open access   Peer reviewed

Time-dependent nanomechanics of cartilage

Lin Han, Eliot H Frank, Jacqueline J Greene, Hsu-Yi Lee, Han-Hwa K Hung, Alan J Grodzinsky and Christine Ortiz
Biophysical journal, v 100(7), pp 1846-1854
06 Apr 2011
DOI: https://doi.org/10.1016/j.bpj.2011.02.031
PMID: 21463599
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1016/j.bpj.2011.02.031View
Published, Version of Record (VoR)Open Access (Publisher-Specific),  Open

Abstract

Animals Biomechanical Phenomena - physiology Cartilage - physiology Cattle Elastic Modulus Extracellular Matrix - metabolism Microscopy, Atomic Force Nanostructures - chemistry Proteoglycans - metabolism Time Factors
In this study, atomic force microscopy-based dynamic oscillatory and force-relaxation indentation was employed to quantify the time-dependent nanomechanics of native (untreated) and proteoglycan (PG)-depleted cartilage disks, including indentation modulus E(ind), force-relaxation time constant τ, magnitude of dynamic complex modulus |E(∗)|, phase angle δ between force and indentation depth, storage modulus E', and loss modulus E″. At ∼2 nm dynamic deformation amplitude, |E(∗)| increased significantly with frequency from 0.22 ± 0.02 MPa (1 Hz) to 0.77 ± 0.10 MPa (316 Hz), accompanied by an increase in δ (energy dissipation). At this length scale, the energy dissipation mechanisms were deconvoluted: the dynamic frequency dependence was primarily governed by the fluid-flow-induced poroelasticity, whereas the long-time force relaxation reflected flow-independent viscoelasticity. After PG depletion, the change in the frequency response of |E(∗)| and δ was consistent with an increase in cartilage local hydraulic permeability. Although untreated disks showed only slight dynamic amplitude-dependent behavior, PG-depleted disks showed great amplitude-enhanced energy dissipation, possibly due to additional viscoelastic mechanisms. Hence, in addition to functioning as a primary determinant of cartilage compressive stiffness and hydraulic permeability, the presence of aggrecan minimized the amplitude dependence of |E(∗)| at nanometer-scale deformation.

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Web of Science research areas
Biophysics
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