Journal article
Tonic and stimulus-evoked nitric oxide production in the mouse olfactory bulb
Neuroscience, v 153(3), pp 842-850
08 Mar 2008
PMID: 18407420
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Nitric oxide (NO) has been long assumed to play a key role in mammalian olfaction. This was based largely on circumstantial evidence, i.e. prominent staining for nitric oxide synthase (NOS) and cyclic GMP or soluble guanylyl cyclase, an effector enzyme activated by NO, in local interneurons of the olfactory bulb. Here we employ innovative custom-fabricated NO micro-sensors to obtain the first direct, time-resolved measurements of NO signaling in the olfactory bulb. In 400 μm thick mouse olfactory bulb slices, we detected a steady average basal level of 87 nM NO in the extracellular space of mitral or granule cell layers. This NO ‘tone’ was sensitive to NOS substrate manipulation (200 μM L-arginine, 2 mM L-NAME) and Mg
2+
modulation of NMDA receptor conductance. Electrical stimulation of olfactory nerve fibers evoked transient (peak at 10 s) increments in NO levels 90 – 100 nM above baseline. In the anesthetized mouse, NO micro-sensors inserted into the granule cell layer detected NO transients averaging 55 nM in amplitude and peaking at 3.4 sec after onset of a 5 sec odorant stimulation. These findings suggest dual roles for NO signaling in the olfactory bulb – tonic inhibitory control of principal neurons, and regulation of circuit dynamics during odor information processing.
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Details
- Title
- Tonic and stimulus-evoked nitric oxide production in the mouse olfactory bulb
- Creators
- Graeme Lowe - Monell Chemical Senses CenterDonald G. Buerk - University of PennsylvaniaJie Ma - Monell Chemical Senses CenterAlan Gelperin - Monell Chemical Senses Center
- Publication Details
- Neuroscience, v 153(3), pp 842-850
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000256042400028
- Scopus ID
- 2-s2.0-43049136544
- Other Identifier
- 991019231746604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences