Journal article
Toward Next-Generation Biohybrid Catalyst Design: Influence of Degree of Polymerization on Enzyme Activity
Bioconjugate chemistry, v 31(3), pp 939-947
18 Mar 2020
PMID: 32078301
Abstract
Due to their capacity to conduct complex organic transformations, enzymes find extensive use in medical and industrial settings. Unfortunately, enzymes are limited by their poor stability when exposed to harsh non-native conditions. While a host of methods have been developed to stabilize enzymes in non-native conditions, recent research into the synthesis of polymer-enzyme biohybrids using reversible deactivation radical polymerization approaches has demonstrated the potential of increased enzymatic activity in both native and non-native environments. In this manuscript, we utilize the enzyme lipase, as a model system, to explore the impact that modulation of grafted polymer molecular weight has on enzyme activity in both aqueous and organic media. We studied the properties of these hybrids using both solution-phase enzyme activity methods and coarse-grain modeling to assess the impact of polymer grafting density and grafted polymer molecular weight on enzyme activity to gain a deeper insight into this understudied property of the biohybrid system.
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Details
- Title
- Toward Next-Generation Biohybrid Catalyst Design: Influence of Degree of Polymerization on Enzyme Activity
- Creators
- Marina Kovaliov - Allegheny General HospitalThaiesha A Wright - Miami UniversityBoyle Cheng - Allegheny General HospitalRobert T Mathers - Pennsylvania State UniversityXiangyu Zhang - Mississippi State UniversityDong Meng - Mississippi State UniversityKatarzyna Szcześniak - Adam Mickiewicz University in PoznańJacek Jenczyk - Adam Mickiewicz University in PoznańStefan Jurga - Adam Mickiewicz University in PoznańDevora Cohen-Karni - Lake Erie College of Osteopathic MedicineRichard C Page - Miami UniversityDominik Konkolewicz - Miami UniversitySaadyah Averick - Allegheny Health Network
- Publication Details
- Bioconjugate chemistry, v 31(3), pp 939-947
- Publisher
- American Chemical Society (ACS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Surgery; Neurosurgery
- Web of Science ID
- WOS:000526399100055
- Scopus ID
- 2-s2.0-85082094512
- Other Identifier
- 991021930452104721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- Chemistry, Organic