Journal article
Toxoplasma gondii induces the secretion of monocyte chemotactic protein-1 in human fibroblasts, in vitro
Molecular and cellular biochemistry, v 209(1-2), pp 79-87
01 Jun 2000
PMID: 10942204
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Secretion of Monocyte Chemotactic Protein-1 (MCP-1) by fibroblasts infected with Toxoplasma gondii was studied in vitro. A significantly higher MCP-1 secretion was observed 24 h after infection by live tachyzoites. Analysis of chemokine mRNA transcripts by RNase protection assay revealed that this MCP-1 secretion seems associated with increased MCP-1 mRNA expression. However, these increased levels of MCP-1 secretion and expression were not obtained after stimulation by heat-killed tachyzoites or parasites pre-treated by a specific inhibitor of phosphatidylcholine-specific phospholipase C (D609). Inhibition of parasite multiplication by pyrimethamine did not modify MCP-1 secretion. Thus, it appeared that the active penetration of T. gondii in cells was of major importance in the induction of MCP-1 secretion. None of the other chemokines studied by RNase protection assay (lymphotactin, RANTES, IP-10, MIP-1α, MIP-1β, IL-8, and I-309) were expressed after infection by live tachyzoites. We also found that MCP-1 secretion induced by live T. gondii is blocked by inhibitors of nuclear factor (NF)-κ activation, ALLN and MG132. Such data indicate that NF-κB could be involved in T. gondii-induced MCP-1 production. MCP-1 secretion may contribute to the recruitment of monocytes and lymphocytes and thus participate in the control of T. gondii infection and in its pathogenesis.[PUBLICATION ABSTRACT]
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Details
- Title
- Toxoplasma gondii induces the secretion of monocyte chemotactic protein-1 in human fibroblasts, in vitro
- Creators
- Marie-pierre Brenier-pinchart - Université Joseph FourierHervé Pelloux - Université Joseph FourierJosiane Simon - Université Joseph FourierJérôme RicardJean-luc BossonPierre Ambroise-thomas - Université Grenoble Alpes
- Publication Details
- Molecular and cellular biochemistry, v 209(1-2), pp 79-87
- Publisher
- Springer Nature B.V
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000087600700010
- Scopus ID
- 2-s2.0-0033933439
- Other Identifier
- 991021229894304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology