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Toxoplasma gondii:Role of the Phosphatidylcholine-Specific Phospholipase C during Cell Invasion and Intracellular Development
Journal article   Peer reviewed

Toxoplasma gondii:Role of the Phosphatidylcholine-Specific Phospholipase C during Cell Invasion and Intracellular Development

Jérôme Ricard, Hervé Pelloux, Anne-Laure Favier, Uwe Gross, Elisabeth Brambilla and Pierre Ambroise-Thomas
Experimental parasitology, v 91(3), pp 231-237
01 Mar 1999
DOI: https://doi.org/10.1006/expr.1998.4353
PMID: 10072325
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

MRC5 fibroblasts Protozoa Apicomplexa RH strain Hepes (N-2-hydroxyethyl piperazine-N′-2-ethanesulfonic acid) Tox oplasma gondii D609 (tricyclodecan-9-yl xanthate) uracil uptake AIDS (acquired immunodeficiency syndrome) TCA (trichloroacetic acid) NO (nitric oxide) E.C.: 3.1.4.3 bradyzoite stage-specific antibody phosphatidylcholine-specific phospholipase C (PC-PLC Prugniaud strain multiplication PBS (phosphate-buffered saline) cyst development TNF-α (tumor necrosis factor-α)
Ricard, J., Pelloux, H., Favier, A.-L., Gross, U., Brambilla, E., and Ambroise-Thomas, P. 1999.Toxoplasma gondii:Role of the phosphati-dylcholine-specific phospholipase C during cell invasion and intracellu lar development.Experimental Parasitology91,231–237. The effect of D609, a specific inhibitor of phosphatidylcholine-specific phospholipase C, was investigated on cyst development of the Prugniaud strain ofToxoplasma gondii in vitro.Following treatment with the inhibitor 24 h after cell infection, cyst development was affected as assessed by staining with the bradyzoite-specific mAb CC2: the CC2-reactive antigen was shown to be differently located (in the wall versus the matrix under control conditions). This correlated with a decrease in parasite multiplication induced by D609. Pretreatment of the parasites with D609 inhibited their entry into the host cells, whereas pretreatment of the host cells enhanced the intracellular multiplication of the para sites, without any effect on cell invasion or cyst formation. Our results suggest a crucial role for phosphatidylcholine-specific phospholipase C in the pathophysiology of toxoplasmosis.

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Parasitology
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