Journal article
Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging
Geomicrobiology journal, v 35(1), pp 81-89
02 Jan 2018
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Metal cofactors are required for many enzymes in anaerobic microbial respiration. This study examined iron, cobalt, nickel, copper, and zinc in cellular and abiotic phases at the single-cell scale for a sulfate-reducing bacterium (Desulfococcus multivorans) and a methanogenic archaeon (Methanosarcina acetivorans) using synchrotron X-ray fluorescence microscopy. Relative abundances of cellular metals were also measured by inductively coupled plasma mass spectrometry. For both species, zinc and iron were consistently the most abundant cellular metals. M. acetivorans contained higher nickel and cobalt content than D. multivorans, likely due to elevated metal requirements for methylotrophic methanogenesis. Cocultures contained spheroid zinc sulfides and cobalt/copper sulfides.
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Details
- Title
- Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging
- Creators
- Jennifer B. Glass (Corresponding Author) - Georgia Institute of TechnologySi Chen - Argonne National LaboratoryKatherine S. Dawson - California Institute of TechnologyDamian R. Horton - Georgia Institute of TechnologyStefan Vogt - Argonne National LaboratoryEllery D. Ingall - Georgia Institute of TechnologyBenjamin S. Twining - Bigelow Laboratory for Ocean SciencesVictoria J. Orphan - California Institute of Technology
- Publication Details
- Geomicrobiology journal, v 35(1), pp 81-89
- Publisher
- Taylor & Francis
- Number of pages
- 9
- Grant note
- DE-SC0004949 / U.S. Department of Energy (10.13039/100000015) NNX14AJ87G / National Aeronautics and Space Administration (10.13039/100000104) NASA Astrobiology Postdoctoral Fellowship,NNX14AJ87G / National Aeronautics and Space Administration (10.13039/100000104) OCE-0939564 / National Science Foundation (10.13039/100000001) 085P1000817 / Michigan Economic Development Corporation (10.13039/100004948) DE-AC02-06CH11357 / U.S. Department of Energy (10.13039/100000015) NNA15BB03A / National Aeronautics and Space Administration OCE-1357375 / National Science Foundation (10.13039/100000001) OCE-1232814 / National Science Foundation (10.13039/100000001)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Environmental Biogeochemistry
- Web of Science ID
- WOS:000428125300009
- Scopus ID
- 2-s2.0-85019580810
- Other Identifier
- 991022207145304721
UN Sustainable Development Goals (SDGs)
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Environmental Sciences
- Geosciences, Multidisciplinary