Journal article
Tracking inflammation in the epileptic rat brain by bi-functional fluorescent and magnetic nanoparticles
Nanomedicine, v 12(5), pp 1335-1345
Jul 2016
PMID: 26964483
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Correct localization of epileptic foci can improve surgical outcome in patients with drug-resistant seizures. Our aim was to demonstrate that systemically injected nanoparticles identify activated immune cells, which have been reported to accumulate in epileptogenic brain tissue. Fluorescent and magnetite-labeled nanoparticles were injected intravenously to rats with lithium-pilocarpine-induced chronic epilepsy. Cerebral uptake was studied ex vivo by confocal microscopy and MRI. Cellular uptake and biological effects were characterized in vitro in murine monocytes and microglia cell lines. Microscopy confirmed that the nanoparticles selectively accumulate within myeloid cells in the hippocampus, in association with inflammation. The nanoparticle signal was also detectable by MRI. The in vitro studies demonstrate rapid nanoparticle uptake and good cellular tolerability. We show that nanoparticles can target myeloid cells in epileptogenic brain tissue. This system can contribute to pre-surgical and intra-surgical localization of epileptic foci, and assist in detecting immune system involvement in epilepsy.
Bi-functional fluorescent and magnetic nanoparticles (MNPs) were used to image epileptogenic tissue in the rat brain. The MNPs were injected intravenously to rats with chronic spontaneous seizures. MNPs selectively accumulated within myeloid cells in the hippocampus, in association with inflammation. The MNP signal was detectable by immunohistochemistry and by MRI. This system can contribute to pre-surgical and intra-surgical localization of epileptic foci, and assist in detecting immune system involvement in epilepsy. [Display omitted]
Metrics
Details
- Title
- Tracking inflammation in the epileptic rat brain by bi-functional fluorescent and magnetic nanoparticles
- Creators
- Emma Portnoy - Hebrew University of JerusalemBoris Polyak - Drexel UniversityDorrit Inbar - Hebrew University of JerusalemGilad Kenan - Hebrew University of JerusalemAhmad Rai - Drexel UniversitySuzanne L. Wehrli - University of PennsylvaniaTimothy P.L. Roberts - University of PennsylvaniaAmeer Bishara - Hebrew University of JerusalemAniv Mann - Hebrew University of JerusalemMiriam Shmuel - Hebrew University of JerusalemKatya Rozovsky - Hadassah Medical CenterGal Itzhak - Hebrew University of JerusalemTamir Ben-Hur - Hebrew University of JerusalemShlomo Magdassi - Hebrew University of JerusalemDana Ekstein - Hebrew University of JerusalemSara Eyal - Hebrew University of Jerusalem
- Publication Details
- Nanomedicine, v 12(5), pp 1335-1345
- Publisher
- Elsevier
- Grant note
- Israel Ministry of Industry and Trade 5R01HL107771 / National Heart, Lung and Blood Institute Prusiner-Abramsky Awards in Basic Clinical Neuroscience 520008095; 510424534 / Drexel-IDR Translational Research Partnership Louis and Bessie Stein Family foundation Brettler Centre for Research in Molecular Pharmacology and Therapeutics
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Surgery
- Web of Science ID
- WOS:000377409100017
- Scopus ID
- 2-s2.0-84963642800
- Other Identifier
- 991019168726404721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Medicine, Research & Experimental
- Nanoscience & Nanotechnology