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Trafficking of conventional dendritic cells into the central nervous system in response to SIV and Morphine creating viral reservoir in follicular DC within cervical lymph nodes of rhesus macaques (VIR9P.1146)
Journal article   Peer reviewed

Trafficking of conventional dendritic cells into the central nervous system in response to SIV and Morphine creating viral reservoir in follicular DC within cervical lymph nodes of rhesus macaques (VIR9P.1146)

Pooja Jain, Artinder Nehra, Rebecca Hollenbach, Divya Sagar, Shannon Callen, Honghong Yao, Shilpa Buch, Zafar Khan and Siddappa Byrareddy
The Journal of immunology (1950), v 194(1_Supplement), pp 215-215.12
01 May 2015
DOI: https://doi.org/10.4049/jimmunol.194.Supp.215.12

Abstract

Abstract Neuroinvasion by HIV leads to neurocognitive diseases and alters the permeability of the BBB. Conventional dendritic cells (cDCs) infiltrating the CNS can potentially encounter HIV from infected perivascular macrophages lining the BBB. They can thereafter carry HIV to cervical lymph nodes (CxLNs), where HIV particles will be transmitted to CD4 T cells and eventually get trapped in CD35+ follicular DCs (fDCs) to create a viral reservoir within the germinal centers. HIV patients using drugs of abuse such as morphine can further compromise the BBB and enhance infectivity of the CNS. Research demonstrating the presence and role of DCs in the CNS and lymphoid organs during HIV infection has not well established. In this respect, we explored the presence of DCs in the brain parenchyma and CxLNs of rhesus macaques infected with SIV and administered with morphine. Cells positive for cDC markers were consistently found in the brain parenchyma of SIV-infected macaques and enhanced within infected macaques given morphine. Examination of CxLNs in SIV-infected macaques confirmed presence of SIV p27+ cDCs, and CD4+ T-cells entering CD20+ germinal centers further trapping of virus within CD35+ fDCs. Ongoing studies are assessing the effect of SIV chronic infection and Morphine on fDC viral entrapment. These results provide first evidence of DC trafficking and containment of SIV particles within fDCs of cervical lymph nodes in the context of NeuroAIDS vis-à-vis drugs of abuse.

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