Files and links (1)
Published, Version of Record (VoR)Open Access (License Unspecified), Open
Journal article
Transcription factor E2F is required for efficient expression of the hamster dihydrofolate reductase gene in vitro and in vivo
Molecular and cellular biology, v 9(11), pp 4994-5002
01 Nov 1989
PMID: 2601705
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The dihydrofolate reductase (DHFR) gene encodes an enzyme important for metabolism and cell growth. We have found multiple DNA-protein interactions within the hamster DHFR gene promoter in vitro. These interactions occur over the consensus binding sites for two eucaryotic transcription factors. Sp1 and E2F. The DHFR E2F consensus site possesses a dyad symmetry and is unique in its location immediately 3' to the major transcription start site. The interaction of E2F with the DHFR promoter has been detected in HeLa nuclear extracts, confirmed by using partially purified E2F, and characterized by both enzymatic and chemical assays of the DNA-protein interaction. A mutation of the E2F recognition sequence which abolishes E2F binding to the DHFR promoter results in a two- to fivefold decrease of in vitro transcriptional activity and a fivefold reduction of DHFR promoter activity in transient-expression assays. Thus, the interaction of E2F with the DHFR promoter is required for efficient expression of the DHFR gene.
Metrics
Details
- Title
- Transcription factor E2F is required for efficient expression of the hamster dihydrofolate reductase gene in vitro and in vivo
- Creators
- M C Blake - Lineberger Cancer Research Center, Department of Pediatrics, University of North Carolina, Chapel Hill 27599-7295J C Azizkhan
- Publication Details
- Molecular and cellular biology, v 9(11), pp 4994-5002
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:A1989AW75200048
- Scopus ID
- 2-s2.0-0024318029
- Other Identifier
- 991020200753104721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology