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Transcription of Hepatitis Delta Virus RNA by RNA Polymerase II
Journal article   Open access   Peer reviewed

Transcription of Hepatitis Delta Virus RNA by RNA Polymerase II

Jinhong Chang, Xingcao Nie, Ho Eun Chang, Ziying Han and John Taylor
Journal of virology, v 82(3), pp 1118-1127
01 Feb 2008
DOI: https://doi.org/10.1128/JVI.01758-07
PMID: 18032511
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1128/JVI.01758-07View
Published, Version of Record (VoR)Open Access (License Unspecified),  Open

Abstract

Genome and Regulation of Viral Gene Expression
Previous studies have indicated that the replication of the RNA genome of hepatitis delta virus (HDV) involves redirection of RNA polymerase II (Pol II), a host enzyme that normally uses DNA as a template. However, there has been some controversy about whether in one part of this HDV RNA transcription, a polymerase other than Pol II is involved. The present study applied a recently described cell system (293-HDV) of tetracycline-inducible HDV RNA replication to provide new data regarding the involvement of host polymerases in HDV transcription. The data generated with a nuclear run-on assay demonstrated that synthesis not only of genomic RNA but also of its complement, the antigenome, could be inhibited by low concentrations of amanitin specific for Pol II transcription. Subsequent studies used immunoprecipitation and rate-zonal sedimentation of nuclear extracts together with double immunostaining of 293-HDV cells, in order to examine the associations between Pol II and HDV RNAs, as well as the small delta antigen, an HDV-encoded protein known to be essential for replication. Findings include evidence that HDV replication is somehow able to direct the available delta antigen to sites in the nucleoplasm, almost exclusively colocalized with Pol II in what others have described as transcription factories.

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Web of Science research areas
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