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Journal article
Transcription of Hepatitis Delta Virus RNA by RNA Polymerase II
Journal of virology, v 82(3), pp 1118-1127
01 Feb 2008
PMCID: PMC2224410
PMID: 18032511
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Previous studies have indicated that the replication of the RNA genome of hepatitis delta virus (HDV) involves redirection of RNA polymerase II (Pol II), a host enzyme that normally uses DNA as a template. However, there has been some controversy about whether in one part of this HDV RNA transcription, a polymerase other than Pol II is involved. The present study applied a recently described cell system (293-HDV) of tetracycline-inducible HDV RNA replication to provide new data regarding the involvement of host polymerases in HDV transcription. The data generated with a nuclear run-on assay demonstrated that synthesis not only of genomic RNA but also of its complement, the antigenome, could be inhibited by low concentrations of amanitin specific for Pol II transcription. Subsequent studies used immunoprecipitation and rate-zonal sedimentation of nuclear extracts together with double immunostaining of 293-HDV cells, in order to examine the associations between Pol II and HDV RNAs, as well as the small delta antigen, an HDV-encoded protein known to be essential for replication. Findings include evidence that HDV replication is somehow able to direct the available delta antigen to sites in the nucleoplasm, almost exclusively colocalized with Pol II in what others have described as transcription factories.
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Details
- Title
- Transcription of Hepatitis Delta Virus RNA by RNA Polymerase II
- Creators
- Jinhong Chang - Fox Chase Cancer CenterXingcao Nie - Fox Chase Cancer CenterHo Eun Chang - Fox Chase Cancer CenterZiying Han - Fox Chase Cancer CenterJohn Taylor - Fox Chase Cancer Center
- Publication Details
- Journal of virology, v 82(3), pp 1118-1127
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000252514000006
- Scopus ID
- 2-s2.0-38349133687
- Other Identifier
- 991020547434904721
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- Web of Science research areas
- Virology