Journal article
Transcription through the HIV-1 nucleosomes: Effects of the PBAF complex in Tat activated transcription
Virology (New York, N.Y.), v 405(2), pp 322-333
30 Sep 2010
PMID: 20599239
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The SWI/SNF complex remodels nucleosomes, allowing RNA Polymerase II access to the HIV-1 proviral DNA. It has not been determined which SWI/SNF complex (BAF or PBAF) remodels nucleosomes at the transcription start site. These complexes differ in only three subunits and determining which subunit(s) is required could explain the regulation of Tat activated transcription. We show that PBAF is required for chromatin remodeling at the nuc-1 start site and transcriptional elongation. We find that Baf200 is required to ensure activation at the LTR level and for viral production. Interestingly, the BAF complex was observed on the LTR whereas PBAF was present on both LTR and Env regions. We found that Tat activated transcription facilitates removal of histones H2A and H2B at the LTR, and that the FACT complex may be responsible for their removal. Finally, the BAF complex may play an important role in regulating splicing of the HIV-1 genome.
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Details
- Title
- Transcription through the HIV-1 nucleosomes: Effects of the PBAF complex in Tat activated transcription
- Creators
- Rebecca Easley - George Mason UniversityLawrence Carpio - George Mason UniversityLuke Dannenberg - George Mason UniversitySoyun Choi - George Mason UniversityDowser Alani - George Mason UniversityRachel Van Duyne - George Mason UniversityIrene Guendel - George Mason UniversityZachary Klase - National Institutes of HealthEmmanuel Agbottah - George Mason UniversityKylene Kehn-Hall - George Mason UniversityFatah Kashanchi - George Mason University
- Publication Details
- Virology (New York, N.Y.), v 405(2), pp 322-333
- Publisher
- Elsevier
- Number of pages
- 12
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000281130500007
- Scopus ID
- 2-s2.0-77955660383
- Other Identifier
- 991021902506104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Virology