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Transcriptional Activity of the Herpes Simplex Virus Genome during Establishment, Maintenance, and Reactivation of in vitro Virus Latency
Journal article   Peer reviewed

Transcriptional Activity of the Herpes Simplex Virus Genome during Establishment, Maintenance, and Reactivation of in vitro Virus Latency

Adrienne C Scheck, Brian Wigdahl and Fred Rapp
Intervirology, v 30(3), pp 121-136
1989
DOI: https://doi.org/10.1159/000150084
PMID: 2473963
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Original Paper
We previously have described a model of in vitro herpes simplex virus (HSV) latency in which latent infection was (i) established with human leukocyte interferon (IFN-α) in combination with (¾)-5-(2-bromovinyl)-2’-deoxyuridine (BVDU) or 9-[(2-hydroxyethoxy)methyl]guanine (acyclovir); (ii) maintained after termination of combined inhibitor treatment by incubation at 40.5°, and (iii) reactivated by either reducing the incubation temperature to 37° or by superinfecting at the elevated temperature with human cytomegalo-virus (HCMV). We now report the use of this system to examine the transcriptional activity of the HSV genome during establishment, maintenance, and reactivation of HSV latency in vitro. Numerous species of virus-specific polyadenylated RNAs were present during the first 3 days of combined BVDU and IFN-α treatment of HSV type 1 (HSV-1)-infected human fetus lung fibroblast cells. However, after 7 days of combined inhibitor treatment, only a very small quantity of virus-specific RNA could be detected utilizing the short unique region of the HSV-1 genome as probe. After terminating combined BVDU and IFN-α treatment and increasing the temperature from 37 to 40.5° on day 7 after infection, virus-specific RNA was undetectable by RNA blot hybridization analysis; however, a small amount of HSV-specific RNA was detected in 2% of the cells by in situ hybridization. The HSV-1 transcriptional products produced after HCMV superinfection in the presence of selected inhibitors of macromolecular synthesis also were examined and demonstrated that the efficient activation of HSV-1 immediate-early gene transcription required the expression of not only immediate-early HCMV gene product(s), but also at least a subset of early-late gene products.

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Web of Science research areas
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