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Journal article
Transcriptome analysis of embryonic and adult sensory axons reveals changes in mRNA repertoire localization
RNA (Cambridge), v 17(1), pp 85-98
01 Jan 2011
PMID: 21098654
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
mRNAs are transported, localized, and translated in axons of sensory neurons. However, little is known about the full repertoire of transcripts present in embryonic and adult sensory axons and how this pool of mRNAs dynamically changes during development. Here, we used a compartmentalized chamber to isolate mRNA from pure embryonic and adult sensory axons devoid of non-neuronal or cell body contamination. Genome-wide microarray analysis reveals that a previously unappreciated number of transcripts are localized in sensory axons and that this repertoire changes during development toward adulthood. Embryonic axons are enriched in transcripts encoding cytoskeletal-related proteins with a role in axonal outgrowth. Surprisingly, adult axons are enriched in mRNAs encoding immune molecules with a role in nociception. Additionally, we show Tubulin-beta3 (Tubb3) mRNA is present only in embryonic axons, with Tubb3 locally synthesized in axons of embryonic, but not adult neurons where it is transported, thus validating our experimental approach. In summary, we provide the first complete catalog of embryonic and adult sensory axonal mRNAs. In addition we show that this pool of axonal mRNAs dynamically changes during development. These data provide an important resource for studies on the role of local protein synthesis in axon regeneration and nociception during neuronal development.
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- Title
- Transcriptome analysis of embryonic and adult sensory axons reveals changes in mRNA repertoire localization
- Creators
- Laura F. Gumy - University of CambridgeGiles S. H. Yeo - Addenbrooke's HospitalYi-Chun Loraine Tung - University of CambridgeKrishna H. Zivraj - University of CambridgeDianna Willis - Burke Medical Research InstituteGiovanni Coppola - University of California, Los AngelesBrian Y. H. Lam - Addenbrooke's HospitalJeffery L. Twiss - Drexel UniversityChristine E. Holt - Univ Cambridge, Dept Physiol Dev & Neurosci, Cambridge CB2 3DY, EnglandJames W. Fawcett - Univ Cambridge, Ctr Brain Repair, Cambridge CB2 0PY, England
- Publication Details
- RNA (Cambridge), v 17(1), pp 85-98
- Publisher
- Cold Spring Harbor Lab Press, Publications Dept
- Number of pages
- 14
- Grant note
- G0501592 / MRC; UK Research & Innovation (UKRI); Medical Research Council UK (MRC) G0600717B; G0501592 / Medical Research Council; UK Research & Innovation (UKRI); Medical Research Council UK (MRC) FP7-HEALTH-2009-241592 EurOCHIP / EU; European Union (EU) MRC Centre for Obesity and Related metabolic Disorders (CORD); UK Research & Innovation (UKRI); Medical Research Council UK (MRC) UK Medical Research Council (MRC); UK Research & Innovation (UKRI); Medical Research Council UK (MRC)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000285338200010
- Scopus ID
- 2-s2.0-78650474986
- Other Identifier
- 991021892106504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology