Journal article
Transcriptomic responses of peripheral blood leukocytes to cardiac surgery after acute inflammation, and three months recovery
Genomics (San Diego, Calif.), v 116(5), 110878
01 Sep 2024
PMID: 38851465
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Traumatic perioperative conditions may trigger early systemic responses, activate leukocytes and reprogram the immune system. We hypothesize that leukocyte activation may not revert to pre-surgical states, and that protracted activation may emerge with increased risks of comorbidities. We tested this concept by examining the transcriptomes of monocytes and T cells in a representative observational cohort of patients (n = 13) admitted for elective cardiac surgery. Transcriptomes in T cells and monocytes were compared from before surgery (t0), and monocytes were analyzed longitudinally after acute (t24hr), and convalescent (t3m) time points. Monocytes and T cells expressed distinct transcriptomes, reflected by statistically significant differential expression of 558 T cell related genes. Monocytes expressed genes related to protein degradation and presented atypical activation of surface markers and cytoplasmic functions over time. Additionally, monocytes exhibited limited transcriptomic heterogeneity prior to surgery, and long-term patterns of gene expression associated with atherosclerosis showed three temporally distinct signatures. These data establish that post-cardiac surgery transcriptomes of monocytes differ even at three months compared to baselines, which may reflect latent (‘smoldering’) inflammation and persistent progression of tissue degenerative processes that should inform clinical care.
•The transcriptome of monocytes does not recover to the presurgical state even in seemingly recovered patients at 3 months after surgery with a protracted-expression of genes seen in atherosclerosis and neurodegeneration.•The heterogeneity of the monocyte transcriptome is much more diverse before surgery with significantly less diversity afterwards.•Monocyte transcriptome has highly individualized trajectories in patients in response to supramaximal iatrogenic surgical stress.
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Details
- Title
- Transcriptomic responses of peripheral blood leukocytes to cardiac surgery after acute inflammation, and three months recovery
- Creators
- Eric A. Lewallen - Hampton UniversityDa Liu - China Medical UniversityJake Karwoski - Drexel UniversityWilson Y. Szeto - University of PennsylvaniaAndre J. van Wijnen - University of VermontKrzysztof Laudanski - Mayo Clinic in Arizona
- Publication Details
- Genomics (San Diego, Calif.), v 116(5), 110878
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:001258815800001
- Scopus ID
- 2-s2.0-85196179623
- Other Identifier
- 991022202507804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Genetics & Heredity