Journal article
Transduction of Well-Differentiated Airway Epithelium by Recombinant Adeno-Associated Virus Is Limited by Vector Entry
Journal of virology, v 73(7), pp 6085-6088
Jul 1999
PMID: 10364362
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The limitations of adeno-associated virus (AAV)-mediated vectors for lung-directed gene transfer were investigated by using differentiated human respiratory epithelium in air-liquid interface cultures. Transduction efficiency was high in undifferentiated cells and was enhanced in well-differentiated cells after basolateral application of the vector or after apical application following disruption of tight junctions or pretreatment of the cultures with glycosidases. These results indicate that transduction of airway epithelia by AAV vectors is limited by entry and reinforce the importance of a physical barrier on the airway surface.
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Details
- Title
- Transduction of Well-Differentiated Airway Epithelium by Recombinant Adeno-Associated Virus Is Limited by Vector Entry
- Creators
- Robert Bals - Departments of Medicine and Molecular and Cellular Engineering, Institute for Human Gene Therapy, and The Wistar InstituteWeidong Xiao - Departments of Medicine and Molecular and Cellular Engineering, Institute for Human Gene Therapy, and The Wistar InstituteNianli Sang - Departments of Medicine and Molecular and Cellular Engineering, Institute for Human Gene Therapy, and The Wistar InstituteDaniel J Weiner - Departments of Medicine and Molecular and Cellular Engineering, Institute for Human Gene Therapy, and The Wistar InstituteRupalie L Meegalla - Departments of Medicine and Molecular and Cellular Engineering, Institute for Human Gene Therapy, and The Wistar InstituteJames M Wilson - Departments of Medicine and Molecular and Cellular Engineering, Institute for Human Gene Therapy, and The Wistar Institute
- Publication Details
- Journal of virology, v 73(7), pp 6085-6088
- Series
- Note
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000080813500093
- Scopus ID
- 2-s2.0-0032992225
- Other Identifier
- 991014878178204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Virology