Journal article
Transforming growth factor- beta 1 (TGF- beta 1) regulates ATDC5 chondrogenic differentiation and fibronectin isoform expression
Journal of cellular biochemistry, v 95(4), pp 750-762
01 Jan 2005
PMID: 15832361
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Regulated splicing of fibronectin (FN) occurs during the mesenchymal to chondrocyte transition and ultimately results in the relative enrichment of an extra domain B (EDB) exon-containing FN isoform with the suggestion that FN isoforms may play a functional role in chondrogenesis. Promotion of chondrogenesis can also be achieved by treatment with transforming growth factor- beta (TGF- beta ), which also regulates FN isoform expression. We have examined the effects of TGF- beta treatment on the assumption of the chondrogenic phenotype in the teratoma-derived cell line ATDC5 and tested whether these effects on chondrogenesis are paralleled by appropriate changes in FN isoform expression. ATDC5 cells were maintained in a pre-chondrogenic state and, in this state, treated with 10 ng/ml TGF- beta . The cells started to elaborate a matrix rich in sulfated proteoglycans, such that within the first 12 days of culture, TGF- beta 1 treatment appeared to slightly accelerate early acquisition of an Alcian blue-stained matrix, and caused a dose-and time- dependent decrease in collagen type I expression; changes in collagen type II expression were variable. At later times, cells treated with TGF- beta became indistinguishable from those of the controls. Interestingly, TGF- beta treatment caused a significant dose-and time-dependent decrease in the proportion of FN containing the extra domain A (EDA) and the EDB exons. These data suggest that TGF- beta induces the early stages of chondrogenic maturation in this pre-chondrogenic line and that TGF- beta treatment increases expression of FN isoforms that lack the EDA and EDB exons. Published 2005 Wiley-Liss, Inc.
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Details
- Title
- Transforming growth factor- beta 1 (TGF- beta 1) regulates ATDC5 chondrogenic differentiation and fibronectin isoform expression
- Creators
- Fei HanChristopher S AdamsZhuliang TaoCharlene J WilliamsRaihana ZakaRocky S TuanPamela A NortonNoreen J Hickok
- Publication Details
- Journal of cellular biochemistry, v 95(4), pp 750-762
- Publisher
- Wiley
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000229839600010
- Scopus ID
- 2-s2.0-24744456615
- Other Identifier
- 991014877893204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology