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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Transgene expression within the spinal cord of hTH-eGFP rats
Journal of chemical neuroanatomy, v 109, 101853
Nov 2020
PMID: 32771532
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
•A11 diencephalospinal pathways express reporter gene in a hTH-eGFP transgenic rat•Spinal tyrosine hydroxylase (TH)+ interneurons do not express GFP•Supraspinal noradrenergic projections and afferents of TH+ sensory neurons are not tagged•Ectopic GFP expression emerges in the dorsal root ganglionic (DRG) neurons
The enzyme tyrosine hydroxylase (TH) is broadly expressed in catecholaminergic neurons. In the spinal cord, four components contain TH, including A11 diencephalospinal dopaminergic (DA-ergic) pathways, intraspinal DA-related neurons, supraspinal noradrenergic projections, and afferents of TH-expressing sensory neurons. A human TH-enhanced green fluorescent protein (hTH-eGFP) transgenic rat was recently developed to tag TH+ profiles in the nervous system for visualization. Using immunostaining, we found that only A11 pathways express GFP whereas the other 3 components do not in the spinal cord. Thus, this may suggest a genetic difference among these TH+ elements even though they produce the same protein.
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Details
- Title
- Transgene expression within the spinal cord of hTH-eGFP rats
- Creators
- Shunyi Zhao - Drexel UniversityShaoping Hou - Drexel University
- Publication Details
- Journal of chemical neuroanatomy, v 109, 101853
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000582364400016
- Scopus ID
- 2-s2.0-85089192949
- Other Identifier
- 991019167745404721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences