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Journal article
Transition of Serotype 35B Pneumococci From Commensal to Prevalent Virulent Strain in Children
Frontiers in cellular and infection microbiology, v 11, pp 744742-744742
26 Oct 2021
PMID: 34765566
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In our community-based prospective cohort study in young children, we observed a significant increase in pneumococcal serotype 35B nasopharyngeal (NP) commensal colonization during the 2011-2014 timeframe, but these strains were not associated with disease. Beginning in 2015 and continuing through to the present, the serotype 35B virulence changed, and it became the dominant bacteria isolated and associated with pneumococcal acute otitis-media (AOM) in our cohort. We performed comparative analyses of 250 35B isolates obtained from 140 children collected between 2006 and 2019. Changes in prevalence, clonal-complex composition, and antibiotic resistance were analyzed. Seventy-two (29%) of 35B isolates underwent whole-genome sequencing to investigate genomic changes associated with the shift in virulence that resulted in increased rates of 35B-associated AOM disease. 35B strains that were commensals and AOM disease-causing were mainly associated with sequence type (ST) 558. Antibiotic concentrations of beta-lactams and ofloxacin necessary to inhibit growth of 35B strains rose significantly (2006-2019) (p<0.005). However, only isolates from the 35B/ST558 showed significant increases in MIC50 of penicillin and ofloxacin between the years 2006-2014 and 2015-2019 (p=0.007 and p<0.0001). One hundred thirty-eight SNPs located in 34 different genes were significantly associated with post-2015 strains. SNPs were found in nrdG (metal binding, 10%); metP and metN (ABC transporter, 9%); corA (Mg2+ transporter, 6%); priA (DNA replication, 5%); and on the enzymic gene ldcB (LD-carboxypeptidase, 3%). Pneumococcal serotype 35B strains was a common NP commensal during 2010-2014. In 2015, a shift in increasing number of AOM cases occurred in young children caused by 35B, that was associated with changes in genetic composition and antibiotic susceptibility.
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Details
- Title
- Transition of Serotype 35B Pneumococci From Commensal to Prevalent Virulent Strain in Children
- Creators
- Naoko Fuji - Rochester General Health SystemMichael Pichichero - Rochester General Health SystemRachel L. Ehrlich - Drexel UniversityJoshua Chang Mell - Drexel UniversityGarth D. Ehrlich - Drexel UniversityRavinder Kaur - Rochester General Health System
- Publication Details
- Frontiers in cellular and infection microbiology, v 11, pp 744742-744742
- Publisher
- Frontiers Media Sa
- Number of pages
- 13
- Grant note
- R01-DC 0428 / NIDCD; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Deafness & Other Communication Disorders (NIDCD) R01-DC008671 / NIH NIDCD; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Deafness & Other Communication Disorders (NIDCD) Sanofi
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000717688400001
- Scopus ID
- 2-s2.0-85118781142
- Other Identifier
- 991019168295904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology
- Microbiology